Trimester-specific associations between extracellular vesicle microRNAs and fetal growth
Trimester-specific associations between extracellular vesicle microRNAs and fetal growth
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DOI：
10.1080/14767058.2021.2000598
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发表时间：
2021-11-12
影响因子：
1.8
通讯作者：
Miyasaka, Naoyuki
中科院分区：
文献类型：
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作者：
Fudono, Ayako;Imai, Chihiro;Miyasaka, Naoyuki
目的胎盘源性细胞外囊泡及其运载物，尤其是microRNA（EV-miRNAs），可能参与胎儿和胎盘的发育。在妊娠期间，几种母体血液EV-miRNA（包括胎盘来源的miRNA）的水平在个体之间变化并且在整个妊娠期间变化。然而，这些miRNAs对胎儿生长和孕期特异性的影响尚未完全阐明。本研究的目的是验证两种细胞外囊泡（EV）-miRNA（miR-127 - 3p和miR-26 b-5p）的血清水平可能参与胎儿胎盘调节，其将以三个月特异性方式与胎儿生长显著相关的假设。材料和方法这是一个单中心的出生队列的母亲血清样本，在第二和第三个三个月。为了尽量减少混杂因素的影响，分析仅限于单胎阴道分娩，因此本研究纳入了27名受试者。使用膜亲和方法分离EV RNA，并使用RT-qPCR方法测量miR-127 - 3p和miR-26 b-5p的相对表达水平，其中miR-484作为对照。使用线性回归模型评估两种EV-miRNA与胎儿和胎盘生长之间的关联，并在两个孕期之间进行比较。结果EV-miR-127 - 3p水平在孕中期与出生胎龄体重（BWGA）和胎盘胎龄体重（PWGA）的z值呈负相关，但在孕晚期与之无关。EV-miR-26 b-5p水平与孕中期出生体重呈正相关，但这种相关性在孕晚期减弱。结论循环miRNA水平与胎儿和胎盘生长发育存在一定的孕期特异性相关性。EV-miR-127 - 3p和EV-miR-26 b-5p在胎儿和胎盘发育中的确切作用需要进一步研究。
Objective Placenta-derived extracellular vesicles and their cargoes, especially microRNAs (EV-miRNAs), may contribute to fetal and placental development. During pregnancy, the levels of several maternal blood EV-miRNAs, including miRNAs of placental origin, vary among individuals and change throughout gestation. However, the effects of these miRNAs on fetal growth and trimester-specificity have not been fully elucidated. The purpose of this study is to test the hypothesis that the serum levels of two extracellular vesicles (EV)-miRNAs (miR-127-3p and miR-26b-5p), which may be involved in fetoplacental regulation, would be significantly associated with fetal growth in a trimester-specific manner. Materials and methods This is a single-center birth cohort of maternal serum samples obtained at both the second and third trimesters. To minimize the influence of confounding factors, the analysis was limited to singleton vaginal deliveries, resulting in 27 participants being included in this study. EV RNAs were isolated using a membrane affinity method, and the relative expression levels of miR-127-3p and miR-26b-5p were measured using the RT-qPCR method with miR-484 as control. The associations between the two EV-miRNAs and fetal and placental growth were evaluated using a linear regression model and compared between the two trimesters. Results EV-miR-127-3p levels tended to correlate inversely with the z-scores of birth weight for gestational age (BWGA) and placental weight for gestational age (PWGA) in the second trimester, but not in the third trimester. EV-miR-26b-5p levels were positively associated with birth weight in the second trimester, but this association was weakened in the third trimester. Conclusion Our results suggest a trimester-specific association of circulating miRNA levels with fetal and placental growth. The precise roles of EV-miR-127-3p and EV-miR-26b-5p in fetal and placental development warrant further investigation.