Cell Migration in Confinement: A Micro-Channel-Based Assay
Cell Migration in Confinement: A Micro-Channel-Based Assay
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DOI：
10.1007/978-1-61779-207-6_28
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Piel, Matthieu
中科院分区：
文献类型：
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作者：
Heuze, Melina L.;Collin, Olivier;Piel, Matthieu
本章介绍了一种方法来研究细胞迁移的微通道，一个封闭的环境，定义明确的几何形状。该测定是对更复杂的3D迁移系统的补充，并且提供了几个优点，即使它没有概括3D迁移的全部复杂性。重要的参数如粘附程度、限制程度、机械性能和几何形状可以彼此独立地变化。该设备是完全兼容的几乎任何类型的光学显微镜和简单的几何形状使得自动化分析非常容易执行，这使得筛选strategy.The章节分为五个部分描述了不同类型的迁移室的设计，通过光刻制造模具，组装室，细胞的加载，最后在活细胞或固定细胞上成像。
This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages even if it does not recapitulate the full complexity of 3D migration. Important parameters such as degree of adhesion, degree of confinement, mechanical properties, and geometry can be varied independently of each other. The device is fully compatible with almost any type of light microscopy and the simple geometry makes automated analysis very easy to perform, which allows screening strategy.The chapters is divided into five parts describing the design of different types of migration chambers, the fabrication of a mold by photolithography, the assembly of the chamber, the loading of cells, and finally the imaging on live or fixed cells.