Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model.
Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model.
复制标题
双层随机排列纳米纤维支架可改善兔关节外模型中腱骨愈合的梯度微结构
DOI：
10.2147/ijn.s165633
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发表时间：
2018
影响因子：
8
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Cai J;Wang J;Ye K;Li D;Ai C;Sheng D;Jin W;Liu X;Zhi Y;Jiang J;Chen J;Mo X;Chen S
肌腱/韧带损伤是常见的运动损伤。临床上，需要修复断裂的肌腱或韧带的骨性附着点，但手术修复后附着点结构不能很好地重建。本研究采用静电纺丝技术制备了双层排列随机支架（ARS），旨在研究其对体内肌腱-骨愈合的影响。采用静电纺丝法成功制备了无规和双层排列-无规丝素纤维聚（L-乳酸-己内酯）（P（LLA-CL））纳米纤维支架。将90只新西兰白色兔随机分为3组（随机支架组、ARS组和对照组），通过手术建立自体跟腱关节外腱-骨愈合模型。组织学评估显示，与RS和对照组相比，ARS显著增加了异染面积，减少了界面宽度，并改善了肌腱-骨界面的胶原成熟和组织化。显微计算机断层扫描显示RS和ARS组的骨隧道面积明显小于对照组。实时荧光定量聚合酶链反应显示，6周时RS组和ARS组骨-移植物界面组织中BMP-2和骨桥蛋白的表达水平均高于对照组。术后6周和12周ARS组Ⅰ型胶原表达水平显著高于RS组和对照组。此外，ARS组具有比RS和对照组更好的极限失效载荷和刚度。ARS可通过诱导新骨形成、增加纤维软骨面积、促进胶原组织化和成熟，有效促进兔关节外模型腱-骨结合，改善梯度显微结构。双层排列随机丝素蛋白/P（LLA-CL）纳米纤维支架被证明是一种很有前途的肌腱-骨愈合生物材料。
Tendon/ligament injuries are common sports injuries. Clinically, the repair of a ruptured tendon or ligament to its bony insertion is needed, but the enthesis structure is not well reestablished following surgical repair. Herein, we fabricated dual-layer aligned-random scaffold (ARS) by electrospinning and aimed to investigate the effect of the scaffold on tendon-to-bone healing in vivo. The random and dual-layer aligned-random silk fbroin poly(L-lactic acid-co-e-caprolactone) (P(LLA-CL)) nanofibrous scaffolds were successfully fabricated by electrospinning methods. Ninety New Zealand white rabbits were randomly divided into three groups (random scaffold [RS], ARS, and control groups), and they were subjected to surgery to establish an extra-articular tendon-to-bone healing model with autologous Achilles tendon. Histological assessment showed that the ARS significantly increased the area of metachromasia, decreased the interface width, and improved collagen maturation and organization at the tendon–bone interface compared with the RS and control groups. Microcomputed tomography analysis showed that the bone tunnel area of RS and ARS groups was significantly smaller than those of the control group. Real-time polymerase chain reaction showed that BMP-2 and osteopontin expression levels of the tissue at the interface between the bone and graft in the RS and ARS groups were higher than those of the control group at 6 weeks. Collagen I expression level of the ARS group was significantly higher than those of the RS and control groups at 6 and 12 weeks. Moreover, the ARS groups had a better ultimate load-to-failure and stiffness than the RS and control groups. ARS could effectively augment the tendon-to-bone integration and improve gradient microstructure in a rabbit extra-articular model by inducing the new bone formation, increasing the area of fibrocartilage, and improving collagen organization and maturation. The dual-layer aligned-random silk fibroin/P(LLA-CL) nanofibrous scaffold is proved to be a promising biomaterial for tendon-to-bone healing.