Localization of Deoxyglucose and Annexin A5 in Experimental Atheroma Correlates with Macrophage Infiltration but not Lipid Deposition in the Lesion
Localization of Deoxyglucose and Annexin A5 in Experimental Atheroma Correlates with Macrophage Infiltration but not Lipid Deposition in the Lesion
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DOI：
10.1007/s11307-010-0389-7
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发表时间：
2011-08-01
影响因子：
3.1
通讯作者：
Tamaki, Nagara
中科院分区：
文献类型：
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作者：
Zhao, Yan;Zhao, Songji;Tamaki, Nagara
本研究的目的是了解斑块中脂质沉积与巨噬细胞含量、巨噬细胞代谢和细胞凋亡的关系。我们比较了载脂蛋白E基因敲除（apoE（-/-））小鼠对2-脱氧-2-氟-D-[C-14]葡萄糖（[C-14]FDG）和[Tc-99 m] HYNIC-膜联蛋白V（[Tc-99 m] annexin A5）的摄取与病变组织学的关系。使用主动脉样本（n = 49）的冷冻切片进行双示踪剂放射自显影，并评价区域示踪剂摄取水平。用Movat's五色染色法对病变进行组织学鉴定。（AHA病变表型），Mac-2染色（巨噬细胞浸润）和油红O染色[C-14]FDG的最高摄取量（3.10 +/-1.50%ID ×千克/平方毫米）和[Tc-99 m]膜联蛋白A5（0.49 +/-0.20%ID ×千克/平方毫米）显示在动脉粥样硬化病变（III型和IV型）。每种示踪剂的摄取与巨噬细胞浸润的相关性均优于与脂质沉积的相关性（[C-14]FDG，r = 0.44 vs. r = 0.14; [Tc-99 m]annexin A5，r = 0.65 vs. r = 0.48）。
The aim of this study was to understand the relationship of lipid deposition to the macrophage content, macrophage metabolism, and apoptosis in plaque. We compared the uptake of 2-deoxy-2-fluoro-D-[C-14]glucose ([C-14]FDG) and [Tc-99m]HYNIC-annexin V ([Tc-99m]annexin A5) with the lesion histology in apolipoprotein E knockout (apoE(-/-)) mice.Male apoE(-/-) mice (n = 9) were injected with [C-14]FDG and [Tc-99m]annexin A5. Cryostat sections of aorta samples (n = 49) were used for dual-tracer autoradiography, and regional tracer uptake levels were evaluated. Lesions were identified histologically with Movat's pentachrome (AHA lesion phenotypes), Mac-2 staining (macrophage infiltration) and Oil Red O staining (lipid deposition).The highest uptakes of [C-14]FDG (3.10 +/- 1.50 %ID x kilogram per square millimeter) and [Tc-99m]annexin A5 (0.49 +/- 0.20 %ID x kilogram per square millimeter) were shown in atheromatous lesions (types III and IV). Each tracer uptake showed better correlation with macrophage infiltration than lipid deposition ([C-14]FDG, r = 0.44 vs. r = 0.14; [Tc-99m]annexin A5, r = 0.65 vs. r = 0.48).Both tracers were concentrated in type III and IV atheromatous lesions which corresponded to macrophage infiltration rather than lipid deposition.