Fluorescent pipettes for optically targeted patch-clamp recordings
Fluorescent pipettes for optically targeted patch-clamp recordings
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DOI：
10.1016/j.neunet.2010.02.004
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发表时间：
2010-08-01
期刊：
影响因子：
7.8
通讯作者：
Ikegaya, Yuji
中科院分区：
文献类型：
--
作者：
Ishikawa, Daisuke;Takahashi, Naoya;Ikegaya, Yuji
靶向膜片钳记录是一种有前途的技术，可以直接解决嵌入神经网络中的特定神经元的生理特性。通常，使用荧光显微镜通过荧光染料或荧光蛋白来可视化神经元。切换到透射光显微镜后，重新识别感兴趣的神经元，并用膜片钳移液器在原位目视接近。这里我们介绍一种更简单的神经元靶向方法。使用涂有荧光团的移液器，荧光标记的神经元和移液器吸头在同一显微镜视野中以相同的荧光波长同时成像，从而神经元甚至其神经突被瞄准，而不会遭受光学色差或机械并发症。我们没有发现涂层荧光团影响移液管或神经元的电特性。这项新技术将广泛用于在线视觉控制下的移液管显微操作。 (C) 2010 Elsevier Ltd. 保留所有权利。
Targeted patch-clamp recordings are a promising technique that can directly address the physiological properties of a specific neuron embedded in a neuronal network. Typically, neurons are visualized through fluorescent dyes or fluorescent proteins with fluorescence microscopy. After switching to transmitted light microscopy, neurons of interest are re-identified and visually approached in situ with patch-clamp pipettes. Here we introduce a simpler method for neuron targeting. With fluorophore-coated pipettes, fluorescently labeled neurons and the pipette tips are simultaneously imaged at the same fluorescence wavelength in the same microscope field, so that the neurons and even their neurites are targeted without suffering from chromatic aberration or mechanical complication in optics. We did not find that the coated fluorophores affected the electric properties of pipettes or neurons. The novel technique will be wildly available for pipette micromanipulation under online visual control. (C) 2010 Elsevier Ltd. All rights reserved.