HOMOLOGY BETWEEN VIRF, THE TRANSCRIPTIONAL ACTIVATOR OF THE YERSINIA VIRULENCE REGULON, AND ARAC, THE ESCHERICHIA-COLI ARABINOSE OPERON REGULATOR
HOMOLOGY BETWEEN VIRF, THE TRANSCRIPTIONAL ACTIVATOR OF THE YERSINIA VIRULENCE REGULON, AND ARAC, THE ESCHERICHIA-COLI ARABINOSE OPERON REGULATOR
复制标题
DOI：
10.1128/jb.171.1.254-262.1989
复制
发表时间：
1989-01-01
影响因子：
3.2
通讯作者：
MICHIELS, T
中科院分区：
文献类型：
--
作者：
CORNELIS, G;SLUITERS, C;MICHIELS, T
毒力强的耶尔森氏菌（鼠疫耶尔森氏菌、假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌）在缺乏钙的富培养基中限制其生长，温度为37度。这种特性被称为钙依赖性，与耶尔森氏菌外膜蛋白（Yops）的分泌和致病性有关。它由一种名为pYV的70千碱基质粒介导。Yops的结构基因（yop基因）以及参与控制其表达的基因（vir基因）已经定位在pYV上。在这次交流中，我们发现virF编码一个转录激活子来控制yop调控。该激活剂是一个30,879道尔顿蛋白，与AraC相关，AraC是大肠杆菌和阿拉伯鼠伤寒沙门氏菌的调节因子。我们还在论文中表明，virF的转录是热依赖性的，可能是自动调节的。因此，virF负责温度对Yops产生的影响。最后，我们发现virF可以独立于钙离子的存在激活yop基因的转录。因此，钙的作用仍未得到解释。
Virulent yersiniae (Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica) restrict their growth at 37.degree.C in rich medium deprived of calcium. This property, called calcium dependency, correlates with the secretion of Yersinia outer membrane proteins (Yops) and with pathogenicity. It is mediated by a 70-kilobase plasmid called pYV. The structural genes of the Yops (yop genes), as well as genes involved in the control of their expression (vir genes), have been localized on pYV. In this communication we show that virF encodes a transcriptional activator controlling the yop regulon. This activator is a 30,879-dalton protein related to AraC, the regulator of the Escherichia coli and Salmonella typhimurium arabinose operons. We also show in this paper that transcription of virF is thermodependent and presumably autoregulated. virF is thus responsible for the effect of temperature on the production of the Yops. Finally, we show that virF activates transcription of the yop genes independently of the presence of calcium ions. The role of calcium therefore remains unaccounted for.