A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis
A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis
复制标题
核糖体 DNA 托管的 microRNA 调节斑马鱼胚胎血管生成
DOI：
10.1007/s10456-019-09663-3
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发表时间：
2019-05-01
期刊：
影响因子：
9.8
通讯作者：
Liu, Dong
中科院分区：
文献类型：
--
作者：
Shi, Yunwei;Duan, Xuchu;Liu, Dong
MicroRNAs （miRNAs）是单链小的非编码rna，长度一般为18-25个核苷酸，是基因表达的抑制因子。mirna由独立的基因编码或由各种不同的RNA物种加工而成。到目前为止，还没有证据表明核糖体dna承载的microRNA与脊椎动物的发育有关。目前，我们通过深度测序分析发现，在斑马鱼内皮细胞中，核糖体DNA中一个高表达的小RNA被预测为一种新的miRNA，命名为miR-ntu1。miRNA通过定制设计的Taqman PCR、Northern Blot和硅分析进行验证。此外，我们证明miR-ntu1通过调节Notch信号在斑马鱼血管生成中发挥关键作用。我们的发现提供了一个值得注意的案例，即核糖体DNA中的miRNA参与了脊椎动物的发育。
MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18-25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.