The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro
The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro
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DOI：
10.1016/j.archoralbio.2010.08.006
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Kao, Richard
中科院分区：
文献类型：
--
作者：
Chen, Yong;Wong, Ricky W. K.;Kao, Richard
目的：研究中药乌梅对口腔正畸托槽单种生物膜模型的体外抗菌效果。材料与方法：采用微量稀释法（MIC）研究了乌梅水提物对浮游变形链球菌（S. mutans）的抑菌作用。采用XTT还原法测定了美国Damon3 MX支架（Ormco， USA）上的变形链球菌生物膜暴露于木梅提取物后的细胞活力。利用荧光显微镜和共聚焦激光扫描显微镜（CLSM）对样品进行可视化。结果：高效液相色谱分析表明，乌梅的主要成分为有机酸类化合物。木梅提取物对浮游变形链球菌的MIC为50 mg/mL。用XTT还原法测定变形链球菌生物膜在不同试验剂作用1 min后的光密度（OD）值，结果表明：250 mg/mL木梅提取物对变形链球菌生物膜的细胞活力< BHI (-) （p < 0.01）。显微镜观察发现，乌梅提取物明显增加了支架表面的死菌数量。结论：乌梅提取物在体外对正畸支架上的变形链球菌生物膜有一定的抑菌作用，可作为口腔抗菌药物应用于正畸患者。(C) 2010 Elsevier Ltd.版权所有。
Objective: The aim of this study was to evaluate the antimicrobial efficacy of Traditional Chinese Medicine Fructus mume on a monospecies-biofilm model established on orthodontic brackets in vitro.Materials and methods: The antimicrobial effect of Fructus mume aqueous extract on the planktonic Streptococcus mutans (S. mutans) was tested by microdilution method (MIC). The cell viability of S. mutans biofilm on Damon3 MX bracket (Ormco, USA) after exposed to Fructus mume extract was quantified by XTT reduction assay. Visualization of the samples was performed by fluorescence microscope and confocal laser scanning microscopy (CLSM).Results: HPLC analysis revealed that the main compounds of Fructus mume are organic acids. The MIC of Fructus mume extract on the planktonic S. mutans was 50 mg/mL. The optical density (OD) values, measured by XTT reduction assay from S. mutans biofilms after 1-min exposure to different test agents, demonstrated that the cell viability of S. mutans biofilms exposed to 250 mg/mL Fructus mume extract < BHI (-) (p < 0.01). Microscope image showed that Fructus mume extract obviously increased the amount of dead bacteria on the surface of bracket.Conclusion: Fructus mume extract showed antimicrobial effect on S. mutans biofilm on orthodontic bracket in vitro which may indicate its potential use as an oral antimicrobial agent for orthodontic patients. (C) 2010 Elsevier Ltd. All rights reserved.