Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues
Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues
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DOI：
10.1007/s11033-007-9199-7
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发表时间：
2009-03
影响因子：
2.8
通讯作者：
W. Zeng;B. Zhou;Hui-rong Liu;Guotao Sun;Bao-sheng Chen
中科院分区：
文献类型：
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作者：
W. Zeng;B. Zhou;Hui-rong Liu;Guotao Sun;Bao-sheng Chen
ATP结合盒转运体A1（ABCA 1）调节血浆高密度脂蛋白（HDL）水平，HDL是一种心血管保护因子。树鼩被认为是一种保护动脉粥样硬化的动物，其特征是血浆中高比例的HDL。分别采用SMART-RACE和Real-Time PCR技术鉴定树鼩 ABCA 1的cDNA克隆和表达。结果表明，树鼩 ABCA 1基因全长7,762 bp，其中编码区6,786 bp，编码2,261个氨基酸，与人ABCA 1基因同源性高达95%。结果表明，树鼩 ABCA 1在多种组织中均有表达，其中肺组织表达量最高，其次为肝、肾、脾和心肌，表达量从高到中依次递增。这种模式与人类ABCA 1的模式部分不同，人类ABCA 1在肾脏和心肌中含量较低。这项工作可能为ABCA 1在树鼩独特的HDL代谢中的作用提供新的线索。
ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was identified using SMART-RACE and Real-Time PCR techniques respectively. The nucleotide sequence of tree shrew ABCA1 covered 7,762 bp, including a 6,786 bp coding region which encoded a 2,261 amino acids protein with the high identity to human ABCA1 (95%). Tree shrew ABCA1 was expressed in various tissues, the highest in lung, followed by liver, kidney, spleen and cardiac muscle in turn from high to medium expression levels. This pattern was partially different from that of human ABCA1 which was low in kidney and cardiac muscle. This work could shed new light on its role of ABCA1 in the distinctive HDL metabolism in tree shrew.