HOW MUCH DO WE KNOW ABOUT THE BOHR EFFECT OF HEMOGLOBIN
HOW MUCH DO WE KNOW ABOUT THE BOHR EFFECT OF HEMOGLOBIN
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DOI：
10.1021/bi00394a001
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发表时间：
1987-10-06
期刊：
影响因子：
2.9
通讯作者：
RUSSU, IM
中科院分区：
文献类型：
--
作者：
HO, C;RUSSU, IM
波尔效应，指的是以下评论：怀曼(1964)；佩鲁茨(1970)；安东尼尼和布鲁诺里(1971)；埃德索尔(1972)；基尔马丁和罗西-贝尔纳迪(1973)。在pH大于6时，Hb的氧亲和力随pH增大而增大(碱性玻尔效应)。相反，氧合作用会导致Hb分子释放氢离子。当pH低于6时，随着pH的降低，02的亲和力增加，Hb分子在氧化过程中吸收H+离子(酸性玻尔效应)。首先，根据氧合血红蛋白(Hb02)或一氧化碳(HbCO)2的pH滴定曲线与脱氧Hb的pH滴定曲线之差进行测定。其次，还可以从02亲和力随pH的变化来测量它。根据所述链接
Bohr effect, refer to the following reviews: Wyman (1964); Perutz (1970); Antonini and Brunori (1971); Edsall (1972); Kilmartin and Rossi-Bernardi (1973). The oxygen affinity ofHb, at pH above 6, increases with pH (the alkaline Bohr effect). Conversely, oxygenation results in therelease of hydrogen ions by the Hb molecule. At pH below 6, the 02 affinity increases with decreasing pH and the Hb molecule absorbs H+ ions upon oxygenation (the acid Bohr effect).The Bohr effect can be measured by two independent methods. First, it can be measured from the difference be-tween the pH titration curves of oxyhemoglobin (Hb02) or (carbonmonoxy) hemoglobin (HbCO) 2 and deoxyhemoglobin (deoxy-Hb). Second, it can also be measured from thechange in 02 affinity as a function of pH. According to the linkage