Structure and specific detection of staphylococcal cassette chromosome mec type VII
Structure and specific detection of staphylococcal cassette chromosome mec type VII
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DOI：
10.1016/j.bbrc.2008.10.009
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发表时间：
2008-12-19
影响因子：
3.1
通讯作者：
Yamamoto, Tatsuo
中科院分区：
文献类型：
--
作者：
Higuchi, Wataru;Takano, Tomomi;Yamamoto, Tatsuo
在台湾社区获得性耐甲氧西林金黄色葡萄球菌多位点序列型（ST） 59中发现的葡萄球菌盒式染色体mec (SCCmec) VII型，全长41,347 bp，两侧分别有19 bp的attL和attR序列。它被插入到ST59金黄色葡萄球菌orfX- orfy （Putative tRNA dihydrouridine synthase）区域orfX 3'端的att位点。SCCmecVII的5'端9911-bp核心区域包含attL和盒式染色体重组酶基因（ccrC8），与其他SCC结构、SCCmercury和来自瑞士的mosaic SCCmec共享，表明其在SCC进化中具有重要作用。中心21245 bp的核心区含有mec复合体（C2b）和另一个ccrC基因（ccrC2），与SCCmecV高度同源。而是通过替换，插入和替换。3′端10,191-bp序列是唯一的。因此，SCCmecVII通过重组和插入出现了。我们开发了用于SCCmecVII特异性检测的Multiplex和实时PCR检测方法。事件。(C) 2008爱思唯尔公司版权所有。
Staphylococcal cassette chromosome mec (SCCmec) type VII, found in community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type (ST) 59 from Taiwan, was 41,347 bp in size and flanked by 19-bp attL and attR sequences. It was inserted into the att site at the 3'-end of orfX in the orfX-orfY (Putative tRNA dihydrouridine synthase) region in ST59 S. aureus. The 5'-end side 9911-bp core region of SCCmecVII, which contained attL and the cassette chromosome recombinase gene (ccrC8), was shared by other SCC Structures, SCCmercury and mosaic SCCmec from Switzerland, indicating its important role in SCC evolution. The central 21,245-bp core region contained mec complex (C2b) and another ccrC gene (ccrC2), and was highly homologous to SCCmecV. but with substitutions, insertion and replacement. The 3'-end side 10,191-bp sequence was unique. Therefore, SCCmecVII has emerged through recombination and insertion Multiplex and real-time PCR assays were developed for specific detection of SCCmecVII. events. (C) 2008 Elsevier Inc. All rights reserved.