Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy.
Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy.
复制标题
使用拉曼光谱对混合层中的粘合剂树脂进行定量分析。
DOI：
10.1002/jbm.a.32692
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Jessop,JulieLP
中科院分区：
文献类型：
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作者：
Zou,Yuan;Armstrong,StevenR;Jessop,JulieLP
目的是通过建立基于拉曼光谱基本原理的方法来确定混合层中粘合剂树脂组分的绝对摩尔浓度。将混合层处理为由胶原蛋白和含有两种单体的粘合剂树脂组成的三组分系统。粘合剂标准样品和用785 nm激发波长获得的拉曼峰面积比用于构建共聚单体相对摩尔浓度和Bis-GMA绝对摩尔浓度的单独校准曲线。由于胶原蛋白和水在指纹区域没有可测量的峰，因此定义了稀释系数Kj来描述它们对拉曼峰面积的影响并计算HEMA的绝对摩尔浓度。使用含有丙酮/乙醇/水的类似系统验证方法学。从混合层的顶部四分之一到中间，Bis-GMA和HEMA的绝对摩尔浓度分别降低87%和83%。另外，Bis-GMA比HEMA更少地渗透混合层，如粘合剂树脂层和混合层的上半部分之间的共聚单体摩尔浓度比降低约20%所示。缺乏完全的单体渗透将进一步挑战牙本质粘合剂粘结寿命。© 2010 Wiley Periodicals，Inc.生物医学材料研究杂志，2010年
The objective was to determine absolute molar concentration of adhesive resin components in the hybrid layer by establishing methods based on Raman spectroscopy fundamentals. The hybrid layer was treated as a three‐component system consisting of collagen and an adhesive resin containing two monomers. Adhesive standard specimens and Raman peak area ratios obtained with a 785 nm excitation wavelength were used to construct separate calibration curves for comonomer relative molar concentration and Bis‐GMA absolute molar concentration. As collagen and water had no measurable peaks in the fingerprint region, a dilution coefficientKjwas defined to describe their impact on Raman peak area and to calculate HEMA absolute molar concentration. Methodology was validated using an analogous system containing acetone/ethanol/water. The absolute molar concentration of Bis‐GMA and HEMA decreased 87% and 83%, respectively, from the top quarter to the middle of the hybrid layer. Additionally, less Bis‐GMA penetrated the hybrid layer than HEMA, as indicated by the ∼20% decrease in comonomer molar concentration ratio between the adhesive resin layer and the top half of the hybrid layer. Lack of complete monomer infiltration will further challenge dentin‐adhesive bond longevity. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010