ACTIVATION OF BLOOD-COAGULATION, FIBRINOLYTIC AND KALLIKREIN SYSTEMS DURING STORAGE OF PLASMA
ACTIVATION OF BLOOD-COAGULATION, FIBRINOLYTIC AND KALLIKREIN SYSTEMS DURING STORAGE OF PLASMA
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DOI：
10.1111/j.1423-0410.1984.tb04136.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.7
通讯作者：
AKERBLOM, O
中科院分区：
文献类型：
--
作者：
BLOMBACK, M;CHMIELEWSKA, J;AKERBLOM, O
本研究涉及血浆在+6 ℃下储存如何影响血液凝固、激肽释放酶和纤维蛋白溶解系统的问题。C.将血液收集到柠檬酸盐磷酸盐葡萄糖腺嘌呤（CPD）或酸性柠檬酸盐葡萄糖（ACD）中，并将血浆样品储存在+6 ℃。C、35天每周取样一次，测定不同系统的各种参数。主要凝血酶抑制剂抗凝血酶III的水平未观察到显著变化。在储存期结束时，纤维蛋白肽A水平显著增加，特别是在ACD血浆中，表明凝血酶活化。纤溶酶原水平没有变化，但抗纤溶酶和尿激酶抑制剂的水平降低，纤维蛋白原降解片段B β的水平增加。15-42，表明纤溶系统的激活。ACD血浆中的抗激肽释放酶活性水平急剧下降; CPD血浆受影响较小。 这种降低与自发蛋白水解活性的增加平行，并与纤维蛋白肽A的增加相关。血浆在+6 ℃下长期储存C导致凝血、纤溶和激肽释放酶系统的激活和抑制物的减少。ACD中的激活比CPD血浆中的激活明显得多。
This study deals with the question of how blood coagulation, kallikrein and fibrinolytic systems are affected by storage of plasma at +6.degree. C. Blood was collected into citrate phosphate dextrose adenine (CPD) or acid citrate dextrose (ACD) and the plasma samples were stored at +6.degree. C for 35 days. Samples were taken at weekly intervals for assays of various parameters of the different systems. No significant changes were observed in the levels of the main thrombin inhibitor, antithrombin III. At the end of the storage period, fibrinopeptide A levels increased markedly, particularly in the ACD plasma, indicating thrombin activation. There was no change in the plasminogen level, but a decrease in the levels of antiplasmin and urokinase inhibitors and an increase in the level of the fibrinogen degradation fragment B.beta. 15-42 were observed, indicating activation of the fibrinolytic system. The level of antikallikrein activity decreased sharply in ACD plasma; CPD plasma was less affected. This decrease was parallel to the increase in spontaneous proteolytic activity and correlated with the increase in fibrinopeptide A. Prolonged storage of plasma at +6.degree. C resulted in the activation of coagulation, fibrinolytic and kallikrein systems and decrease in inhibitors. The activation was much more pronounced in ACD than in CPD plasma.