QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa
QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa
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DOI：
10.1073/pnas.051624298
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发表时间：
2001-02-27
影响因子：
11.1
通讯作者：
Greenberg, EP
中科院分区：
文献类型：
--
作者：
Chugani, SA;Whiteley, M;Greenberg, EP
机会致病菌铜绿假单胞菌使用群体感应信号系统作为毒力基因的全局调节器。存在两个群体感应信号受体和信号发生器对，LasR-Lasl和RhlR-Rhll。最近完成的铜绿假单胞菌基因组测序项目揭示了一个编码信号受体LasR和RhlR同源物的基因。在这里，我们描述了这个基因的作用，我们称之为qscR。qscR基因产物控制群体感应控制的基因表达的时间，并且它在昆虫模型中抑制毒力。我们目前的证据表明，QscR的主要作用是抑制lasl。qscR突变体过早地产生LasI产生的信号，这导致许多群体感应调节基因的过早转录。当喂给黑腹果蝇时，qscR突变体比亲本黑腹果蝇更快地杀死动物。通过QscR对lasl的抑制可以确保群体感应控制的基因在它们无用的环境中不被激活。
The opportunistic pathogenic bacterium Pseudomonas aeruginosa uses quorum-sensing signaling systems as global regulators of virulence genes. There are two quorum-sensing signal receptor and signal generator pairs, LasR-Lasl and RhlR-Rhll. The recently completed P. aeruginosa genome-sequencing project revealed a gene coding for a homolog of the signal receptors, LasR and RhlR. Here we describe a role for this gene, which we call qscR. The qscR gene product governs the timing of quorum-sensing-controlled gene expression and it dampens virulence in an insect model. We present evidence that suggests the primary role of QscR is repression of lasl. A qscR mutant produces the Lasl-generated signal prematurely, and this results in premature transcription of a number of quorum-sensing-regulated genes. When fed to Drosophila melanogaster, the qscR mutant kills the animals more rapidly than the parental P. aeroginosa. The repression of lasl by QscR could serve to ensure that quorum-sensing-controlled genes are not activated in environments where they are not useful.