Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiation
Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiation
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DOI：
10.1016/s0378-4274(02)00439-3
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发表时间：
2003-03-20
影响因子：
3.5
通讯作者：
Aruna, R
中科院分区：
文献类型：
--
作者：
Jagetia, GC;Aruna, R
研究了0、0.25、0.5、1、2和3GyCo-60γ射线对HeLa细胞生长动力学、细胞存活率和微核形成的影响。在照射后20、30和40h，AZT预处理组的细胞生长动力学、细胞存活率和细胞增殖指数均显著低于PBS+照射组。反之，PbS+照射组和AZT+照射组的微核双核细胞(MNBNC)率均呈剂量依赖性增加。在所研究的所有照射后评分时间段，与前一组相比，后一组的MNBNC诱导率显著升高。PbS+照射组和AZT+照射组诱导微核的剂量-效应关系均呈线性关系。通过细胞存活率与MNBNC诱导的相关性来研究其生物学反应。细胞存活率与MNBNC诱导作用呈负相关，且符合线性二次模型。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
The effect of 0.1 muM azidothymidine (AZT) a pyrimidine analogue has been studied on the growth kinetics, cell survival and micronuclei formation in HeLa cells exposed to 0, 0.25, 0.5, 1, 2 and 3 Gy of Co-60 gamma-radiation. The AZT pretreatment resulted in a significant decline in the cell growth kinetics, cell survival and cell proliferation indices when compared with the PBS+irradiation group at 20, 30 and 40 h post-irradiation. Conversely, the frequency of micronucleated binucleate cells (MNBNC) elevated in a dose dependent manner in both PBS + irradiation and AZT + irradiation group. This elevation in MNBNC-induction was significantly higher in the latter when compared with the former group at all post-irradiation scoring time periods studied. The dose-response relationship for micronuclei induction for both the PBS + irrradiation and AZT + irradiation groups was linear. The biological response was studied by correlating the cell survival with MNBNC-induction. The cell survival and MNBNC-induction showed a close but inverse relationship and this relationship gave a best fit on the linear quadratic model. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.