Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms
Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms
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DOI：
10.1128/aac.48.3.774-779.2004
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发表时间：
2004-03-01
影响因子：
4.9
通讯作者：
Sekimizu, K
中科院分区：
文献类型：
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作者：
Hamamoto, H;Kurokawa, K;Sekimizu, K
将对人类致病的细菌（金黄色葡萄球菌、嗜麦芽寡养单胞菌）或真真菌（白色念珠菌、热带念珠菌）注射到家蚕血淋巴中，导致幼虫在2天内死亡。临床上使用的抗生素对感染这些病原体的蚕有治疗作用。通过注射到家蚕血淋巴中获得的50%有效剂量与小鼠报告的剂量一致。家蚕血淋巴内注射万古霉素和卡那霉素效果较好，而口服则不明显。氯霉素，这是有效的口服给药，出现在家蚕血淋巴注射到中肠后不久，而万古霉素没有。分离的中肠膜对万古霉素是不可渗透的。因此，蚕口服万古霉素无效是由于缺乏肠道吸收。
The injection of bacteria (Staphylococcus aureus, Stenotrophomonas maltophilia) or true fungi (Candida albicans, Candida tropicalis) that are pathogenic to humans into the silkworm hemolymph leads to death of the larvae within 2 days. Antibiotics used for clinical purposes have therapeutic effects on silkworms infected with these pathogens. The 50% effective doses obtained by injection into the silkworm hemolymph are consistent with those reported for mice. Injection of vancomycin and kanamycin into the silkworm hemolymph was effective, but oral administration was not. Chloramphenicol, which is effective by oral administration, appeared in the silkworm hemolymph soon after injection into the midgut, whereas vancomycin did not. Isolated midgut membranes were impermeable to vancomycin. Thus, the ineffectiveness of oral administration of vancomycin to silkworms is due to a lack of intestinal absorption.