Components Subcellular Localization: Cell Surface Exposure.
Components Subcellular Localization: Cell Surface Exposure.
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组件亚细胞定位：细胞表面暴露。
DOI：
10.1007/978-1-0716-3445-5_7
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Konovalova,Anna
中科院分区：
文献类型：
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作者：
Konovalova,Anna
革兰氏阴性菌的表面暴露蛋白以整体外膜β桶蛋白和脂蛋白为代表。没有计算方法来预测表面暴露的脂蛋白，因此脂蛋白的拓扑结构必须通过实验测试。本章描述了几种不同但互补的检测表面暴露蛋白的方法：细胞表面蛋白标记，细胞外蛋白酶或抗体的可及性，和SpyTag/SpyCatcher系统。
Surface-exposed proteins of Gram-negative bacteria are represented by integral outer membrane β-barrel proteins and lipoproteins. There are no computational methods to predict surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes several distinct but complementary methods for detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular protease or antibodies, and SpyTag/SpyCatcher system.