Phasing of RecA monomers on quasi-random DNA sequences.
Phasing of RecA monomers on quasi-random DNA sequences.
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RecA 单体在准随机 DNA 序列上的定相。
DOI：
10.1016/s0014-5793(03)00572-6
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
Camerini-Otero,RDaniel
中科院分区：
文献类型：
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作者：
Volodin,AlexanderA;Smirnova,ElenaA;Bocharova,TatjanaN;Camerini-Otero,RDaniel
我们发现，一些任意选择的DNA序列有能力影响RecA单体在RecA-DNA复合物中的定位。RecA单体结合的优先阶段取决于DNA序列及其核苷酸组成。在有限的DNA片段中碱基的简单重排影响RecA单体的定相。另一方面，DNA序列的某些特征干扰特定DNA位点上的定相，这表明存在对天然DNA定相的正调节和负调节机制。RecA单体对DNA的定相的可能作用进行了讨论。
We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA–DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch influences the phasing of RecA monomers. On the other hand, that some features of DNA sequences interfere with the phasing on specific DNA sites demonstrates the existence of mechanisms for both positive and negative regulation of phasing on natural DNAs. The possible role of phasing of RecA monomers on DNA is discussed.