pH-dependent long-term radical scavenging activity of AA-2G and 6-Octa-AA-2G against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation.
pH-dependent long-term radical scavenging activity of AA-2G and 6-Octa-AA-2G against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation.
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AA-2G 和 6-Octa-AA-2G 对 2,2-azinobis(3-乙基苯并噻唑啉-6-磺酸) 自由基阳离子的 pH 依赖性长期自由基清除活性。
DOI：
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发表时间：
2003
影响因子：
2
通讯作者：
I. Yamamoto
中科院分区：
文献类型：
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作者：
J. Takebayashi;A. Tai;I. Yamamoto
采用2，2‘-azinobis(3-ethylbenzothiazoline-6-sulfonic酸自由基阳离子(ABTS(自由基+))脱色实验评价了抗坏血酸(AA)及其两个衍生物2-O-α-D-葡萄糖基-L-抗坏血酸(AA-2G)和2-O-alpha-D-glucopyranosyl-6-O-octanoyl-L-ascorbic酸(6-OCTA-AA-2G)的化学计量比自由基清除活性。AA与ABTS(自由基+)反应迅速，反应在10分钟内完成。AA-2G和6-Octa-AA-2G在pH 4.0及以上的水溶液中清除自由基活性高于pH 3.0的水溶液，而AA在pH 3~6范围内的清除活性无明显差异。在pH 6.0下反应2 h后，AA、AA-2G和6-Octa-AA-2G对ABTS(自由基+)的清除量约为2.0。分别为3.4或3.9个分子。研究表明，在长期反应中，AA-2G和6-Octa-AA-2G猝灭ABTS(自由基+)的量优于AA。
The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS (radical +)) decolorization assay was applied to evaluate the stoichiometric radical scavenging activity of ascorbic acid (AA) and two AA derivatives, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) and 2-O-alpha-D-glucopyranosyl-6-O-octanoyl-L-ascorbic acid (6-Octa-AA-2G). AA rapidly reacted with ABTS (radical +), and the reaction was completed within 10 min. In contrast, AA-2G and 6-Octa-AA-2G continuously reacted with ABTS (radical +), and the reaction was not completed after 2 h. The radical scavenging activity of AA-2G and 6-Octa-AA-2G in aqueous solutions at pH 4.0 and above was higher than that at pH 3.0, whereas AA showed no difference in the pH range 3 to 6. The amounts of ABTS (radical +) scavenged by one molecule of AA, AA-2G and 6-Octa-AA-2G after 2 h of reaction at pH 6.0 were approximately 2.0, 3.4 or 3.9 molecules, respectively. This study demonstrates that the quantity of ABTS (radical +) quenched by AA-2G and 6-Octa-AA-2G is superior to that of AA in a long-term reaction.