ABILITY OF LABORATORY METHODS TO PREDICT IN-USE EFFICACY OF ANTIMICROBIAL PRESERVATIVES IN AN EXPERIMENTAL COSMETIC
ABILITY OF LABORATORY METHODS TO PREDICT IN-USE EFFICACY OF ANTIMICROBIAL PRESERVATIVES IN AN EXPERIMENTAL COSMETIC
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实验室方法预测抗菌防腐剂在实验性化妆品中的使用效果的能力
DOI：
10.1128/aem.60.12.4553-4558.1994
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发表时间：
1994-12-01
影响因子：
4.4
通讯作者：
MADDEN, JM
中科院分区：
文献类型：
--
作者：
FARRINGTON, JK;MARTZ, EL;MADDEN, JM
通过六项微生物挑战测试评估了九种抗菌系统保存实验性水基化妆品配方的能力：美国药典测试；英国药典测试；化妆品、盥洗用品和香水协会测试；快速筛选测试；顺序挑战测试；以及使用后测试。该抗菌系统含有两种对羟基苯甲酸酯和一种季化合物的各种组合和数量，以提供广泛的防腐作用。将获得的结果与在为期 8 周的模拟使用测试中，制剂支持从人类腋窝区域和手指皮肤获得的微生物群落中维持和生长的能力进行比较。在没有统计分析的情况下，所有测试都预测了保存完好或保存不良的配方所获得的结果。快速筛选测试是预测中间保存水平差异的最佳测试。从统计上看，所有测试都是使用测试中保存功效的等效预测因子（P = 0.05）。在 P = 0.10 水平上，只有美国药典、英国药典、快速筛查、化妆品、盥洗用品和香料协会测试具有显着预测性。根据所使用的防腐剂水平进行的测试预测结果与实际使用的测试结果非常吻合（P = 0.01）。微生物含量超标的配方中，总共 20% 含有人类腋窝微生物。不合格产品的保存水平与未使用的对照品的保存水平相似。
The abilities of nine antimicrobial systems to preserve an experimental water-based cosmetic formulation were evaluated by six microbiological challenge tests: the U. S. Pharmacopeia test; the British Pharmacopeia test; the Cosmetic, Toiletry, and Fragrance Association test; the rapid screen test; the sequential challenge test; and the post-use test. The antimicrobial systems contained various combinations and amounts of two parabens and a quaternary compound in order to provide a broad range of preservation. The results obtained were compared with the abilities of the formulations to support maintenance and growth of microorganisms in microfloras obtained from human axilla areas and finger skin during an 8-week simulated in-use test. Without statistical analysis all of the tests predicted the results obtained with well-preserved or poorly preserved formulations. The rapid screen test was the best test for predicting differences at intermediate levels of preservation. Statistically, all of the tests were equivalent predictors of preservation efficacy in the in-use test (P = 0.05). At the P = 0.10 level, only the U.S. Pharmaceopeia, British Pharmacopeia, rapid screen, Cosmetic, Toiletry, and Fragrance Association tests were significantly predictive. The results of prediction by a test, based on the preservative levels used, agreed well with the in-use test results (P = 0.01). A total of 20% of the formulations that contained excessive microbial levels contained human axilla microorganisms. The levels of preservation in failed products were similar to the levels of preservation in unused controls.