A single ortholog of teashirt and tiptop regulates larval pigmentation and adult appendage patterning in Bombyx mori
A single ortholog of teashirt and tiptop regulates larval pigmentation and adult appendage patterning in Bombyx mori
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茶衫和尖顶的单一直系同源物调节家蚕幼虫色素沉着和成虫附肢图案
DOI：
10.1016/j.ibmb.2020.103369
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发表时间：
2020
影响因子：
3.8
通讯作者：
Tan Anjiang
中科院分区：
文献类型：
--
作者：
Zhang Ru;Zhang Zhongjie;Huang Yongping;Qian Airong;Tan Anjiang
teashirt（tsh）和tiptop（tio）是两个同源基因，编码锌指转录因子，在昆虫的生长发育过程中起重要作用。在果蝇（Drosophila melanogaster）中，tsh在胚胎阶段促进躯干节段的身份，并有助于其他组织的图案化。在成年阶段，tsh有助于附属物的规格和图案，包括腿，翅膀和眼睛。虽然tsh会产生多余的作用，但缺乏tsh功能的果蝇也能存活，没有有害的表型。这对基因存在于所有果蝇物种的基因组中，但在其他几种昆虫物种中仅作为单一同源物存在。tsh/tio在扁带瘤蜱（Oncopeltus fasciatus）和赤拟谷盗（Tribolium castaneum）中的功能特征是在成虫阶段指定腿的身份。然而，在鳞翅目昆虫中，包括大量的农业和林业害虫，以及主要的丝绸生产者家蚕，tsh/tio的生物学功能仍然知之甚少。在当前的研究中，我们通过使用CRISPR/Cas9介导的诱变和转座子介导的B中的异位表达对tsh/tio进行功能分析。桑。结果表明，tsh/tio功能的丧失影响了幼虫期的色素沉着和成虫期的附肢模式。RNA-seq分析和随后的q-RT-PCR分析表明，在幼虫阶段，tsh/tio的缺失显著提高了犬尿氨酸3-单加氧酶基因以及黑色素氧化酶相关基因的表达。此外，普遍异位表达的tsh/tio诱导发育迟缓，并最终幼虫致死。这些数据揭示了tsh/tioin控制成体附肢模式的进化保守功能，以及调节B. mori，提供了新的见解如何tsh/tio调节昆虫的生长和发育。
Two paralogous genes,teashirt(tsh) andtiptop(tio), encode zinc-finger transcription factors and play important roles in insect growth and development. In the fruit fly, Drosophila melanogaster,tshpromotes trunk segmental identities and contributes to the patterning of other tissues during the embryonic stage. During the adult stage,tshcontributes to the specification and patterning of appendages, including the leg, wing and eye. Whiletioacts redundantly withtsh, flies lackingtiofunction are viable without deleterious phenotypes. This gene pair is present in the genomes of allDrosophilaspecies but only as a single homologue in several other insect species. InOncopeltus fasciatusand Tribolium castaneum, tsh/tiohas been functionally characterized as specifying the identity of the leg during the adult stage. However, in lepidopteran insects which include large numbers of pests in agriculture and forestry, as well as the major silk producer silkwormBombyxmori, the biological functions oftsh/tioare still poorly understood. In the current study, we performed functional analysis oftsh/tioby using both CRISPR/Cas9-mediated mutagenesis and transposon-mediated ectopic expression inB. mori. The results show that loss oftsh/tiofunction affected pigmentation during the larval stage and appendage pattering during the adult stage. RNA-seq analysis and subsequent q-RT-PCR analysis revealed that depletion oftsh/tiosignificantly elevated the expression of thekynurenine3-monooxygenasegene, as well as melanin synthase-related genes during the larval stage. Furthermore, ubiquitous ectopic expression oftsh/tioinduces developmental retardation and eventually larval lethality. These data reveal evolutionarily conserved functions oftsh/tioin controlling adult appendage patterning, as well as the novel function of regulating larval pigmentation inB. mori, providing novel insights into howtsh/tioregulates insect growth and development.