Improvement of Vascularization of PLGA Scaffolds by Inosculation of In Situ-Preformed Functional Blood Vessels With the Host Microvasculature
Improvement of Vascularization of PLGA Scaffolds by Inosculation of In Situ-Preformed Functional Blood Vessels With the Host Microvasculature
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DOI：
10.1097/sla.0b013e31818fa52f
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发表时间：
2008-12-01
影响因子：
9
通讯作者：
Menger, Michael D.
中科院分区：
文献类型：
--
作者：
Laschke, Matthias W.;Ruecker, Martin;Menger, Michael D.
目的：我们在体内分析了通过原位预形成的微血管网络与宿主微血管系统的吻合是否可以加速植入支架的血液供应的建立。背景：快速血管化对于基于支架的移植组织构建体的存活至关重要。将聚乳酸-乙醇酸支架植入balb/c或绿色荧光蛋白（GFP）的侧腹。转基因小鼠20天，以在支架内原位产生新的微血管网络。然后将预血管化的支架转移到同基因受体小鼠的背侧皮褶室中。非血管化聚乳酸-乙醇酸支架作为对照。使用活体荧光显微镜、组织学和免疫组织化学分析植入物的血管形成、血液灌注和细胞存活超过14天。我们的研究结果表明，预血管化支架的血液灌注的建立显着加速和改善与对照组（6.9 +/- 1.9 pl/s）相比，（136.7 +/- 23.2 pl/s），因为原位预成形的微血管通过与宿主微血管形成互连而得到再灌注。只有在缺乏血液灌注支架植入后的第3至6天内，发现植入物内的细胞凋亡死亡，但在进一步的14天observationperiod.Conclusions：原位预制功能血管的接种是一个很有前途的方法，以改善血液供应植入的组织结构。
Objective: We analyzed, in vivo, whether the establishment of blood supply to implanted scaffolds can be accelerated by inosculation of an in situ-preformed microvascular network with the host microvasculature.Background: A rapid vascularization is crucial for the survival of scaffold-based transplanted tissue constructs.Methods: Poly-lactic-glycolic acid scaffolds were implanted into the flank of balb/c or green fluorescent protein (GFP)-transgenic mice for 20 days to create ill Situ a new microvascular network within the scaffolds. The prevascularized scaffolds were then transferred into the dorsal skinfold chamber of isogeneic recipient mice. Nonvascularized poly-lactic-glycolic acid scaffolds served as controls. Vascularization, blood perfusion, and cell survival of the implants were analyzed over 14 days using intravital fluorescence microscopy, histology, and immunohistochemistry.Results: Our results demonstrate that establishment of blood perfusion of prevascularized scaffolds is significantly accelerated and improved (136.7 +/- 23.2 pl/s) when compared with controls (6.9 +/- 1.9 pl/s), because the in situ-preformed microvessels were reperfused by forming interconnections to the host microvasculature. Apoptotic cell death within the implants was found only during the first 3 to 6 days after scaffold implantation during lack of blood perfusion, but not during the further 14-day observation period.Conclusions: Inosculation of in situ-preformed functional blood vessels represents a promising approach to improve the blood supply to implanted tissue constructs.