Effect of ectopic expression of the eutypine detoxifying gene Vr-ERE in transgenic apple plants
Effect of ectopic expression of the eutypine detoxifying gene Vr-ERE in transgenic apple plants
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DOI：
10.1007/s11240-010-9904-4
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发表时间：
2011-07-01
影响因子：
3
通讯作者：
Loridon, K.
中科院分区：
文献类型：
--
作者：
Chevreau, E.;Dupuis, F.;Loridon, K.
开发不含抗生素抗性基因的替代选择系统是生产更安全和更可接受的转基因植物的关键问题。Eutypine是由Eutypa lata产生的毒素，Eutypa lata是葡萄藤Eutypa dieback的病原体，其在绿豆（Vigna radiata）中通过基因Vr-ERE解毒。许多含有醛基的植物毒性化合物可以作为Vr-ERE酶的底物。本工作的目的是评估Vr-ERE在转基因苹果植物中过表达的影响，作为替代选择系统发展的第一步。在卡那霉素选择下，从栽培品种绿袖中产生表达Vr-ERE的有活力的转基因苹果克隆。虽然Vr-ERE转基因通常在RNA和蛋白质水平表达，但在这些克隆中，在一系列潜在底物上测试的醛还原酶活性的增加非常低。与非转基因对照相比，它们都没有显示出对有毒醛类的耐受性显著增加。这项工作与转基因苹果植物过表达的解毒基因Vr-ERE说明了一些困难，在开发一个替代的选择压力。
The development of alternative selection systems without antibiotic resistance genes is a key issue to produce safer and more acceptable transgenic plants. Eutypine is a toxin produced by Eutypa lata, the causal agent of eutypa dieback of grapevine, which is detoxified in mung bean (Vigna radiata) by the gene Vr-ERE. Many phytotoxic compounds containing an aldehyde group can act as substrates for the Vr-ERE enzyme. The aim of the present work was to evaluate the effects of the overexpression of Vr-ERE in transgenic apple plants, as a first step towards the development of an alternative selection system. Viable transgenic apple clones expressing Vr-ERE were produced from the cultivar Greensleeves under kanamycin selection. Although the Vr-ERE transgene was normally expressed at the RNA and protein levels, the increase in aldehyde reductase activity tested on a range of potential substrates was very low in these clones. None of them revealed a significant increase in tolerance to toxic aldehydes compared to their non-transgenic control. This work with transgenic apple plants overexpressing the detoxifying gene Vr-ERE illustrates some of the difficulties in developing an alternative selection pressure.