Transfer of antibiotic resistance genes between yeast and mammalian cells under conditions favoring cell fusion
Transfer of antibiotic resistance genes between yeast and mammalian cells under conditions favoring cell fusion
复制标题
在有利于细胞融合的条件下在酵母和哺乳动物细胞之间转移抗生素抗性基因
DOI：
10.1007/bf01560657
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Balls
中科院分区：
文献类型：
--
作者：
M. Ward;R. Scott;M. Davey;R. Clothier;E. Cocking;M. Balls
Antibiotic resistance to G418 has been transferred into Chinese hamster cell lines via a plasmid vector. The same plasmid, which also contained the Leu2 gene, has been used to transform Leu2− yeast (strain MCI6) to leucine prototrophy. Subsequent fusion between transformed yeast and untransformed hamster cells demonstrated that plasmid DNA could be transferred and its genes expressed within the mammalian cell genome. The fusion of transformed hamster cells with untransformed MC16 yeast cells demonstrated that DNA integrated within the mammalian cell genome could be transferred to correct the Leu2 deficiency and also confer G418 resistance on some yeast colonies.
DOI：
10.1073/pnas.79.2.422
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LOYTER, A;SCANGOS, GA;RUDDLE, FH
通讯作者：
RUDDLE, FH