Cinnamic Acid (CINN) Induces Apoptosis and Proliferation in Human Nasopharyngeal Carcinoma Cells
Cinnamic Acid (CINN) Induces Apoptosis and Proliferation in Human Nasopharyngeal Carcinoma Cells
复制标题
肉桂酸 (CINN) 诱导人鼻咽癌细胞凋亡和增殖
DOI：
10.1159/000452572
复制
发表时间：
2016
影响因子：
--
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Qi Guangying;Chen Jian;Mi Sisi;Shao Wenhua;Ling Jiapu;Qi Guangying;Chen Jian;Shi Changrong;Wang Yukun;Yu Xiangyuan;Ma Yili;Huang Jian;Huang J
背景/目的：肉桂（CINN）是传统中药肉桂的主要成分。本研究的目的是观察CINN对鼻咽癌细胞增殖和凋亡的影响，并探讨其分子机制。材料和方法：用不同浓度的CINN处理CNE 2人鼻咽癌细胞。采用MTT法和流式细胞仪检测CINN对CNE 2鼻咽癌细胞增殖和凋亡的影响。此外，进行蛋白质印迹以分析许多细胞周期和凋亡相关蛋白的表达。结果：不同浓度的CINN作用于CNE 2细胞不同时间后，细胞增殖均受到明显抑制。CINN的抑制作用呈浓度和时间依赖性。流式细胞仪分析显示，2 mmol/L CINN具有明显的细胞凋亡诱导作用。Western blot结果显示，2 mmol/L CINN处理后，CNE 2细胞KLF 6、Fas-L、Bax、P53和caspase-3蛋白表达明显增加，而Bcl-2和cyclin D1蛋白表达明显减少。结论：CINN抑制CNE 2细胞增殖，诱导其凋亡。因此，CINN具有潜在的抗肿瘤作用。
Background/Aims: CINN is the main ingredient of the traditional Chinese medicine cinnamon. The purpose of the present study was to investigate the effects of CINN on the proliferation and apoptosis of NPC cells and to elucidate the underlying molecular mechanisms. Materials and Methods: CNE2 human NPC cells were treated with various CINN concentrations. The effects of CINN on the proliferation and apoptosis of CNE2 NPC cells were examined using the MTT assay and flow cytometric analysis. Additionally, western blotting was performed to analyze the expression of a number of cell cycle- and apoptosis-related proteins. Results: The proliferation of CNE2 cells was significantly inhibited after treatment with different CINN concentrations for various lengths of time. The inhibitory effect of CINN was concentration-and time-dependent. Flow cytometric analysis showed that 2 mmol/L CINN displayed a significant apoptosis-inducing effect. The western blot analysis results showed that KLF6, Fas-L, Bax, P53 and caspase-3 protein expression was drastically increased in the CNE2 cells after treatment with 2 mmol/L CINN, whereas Bcl-2 and cyclin D1 protein expression was markedly reduced. Conclusion: CINN inhibits the proliferation and induces the apoptosis of CNE2 cells. Therefore, CINN possesses a potential anti-tumor effect.