Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media
Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media
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DOI：
10.1007/s11427-010-4119-9
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发表时间：
2011-01-01
影响因子：
9.1
通讯作者：
Yang XiaoHong
中科院分区：
文献类型：
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作者：
Zhou Na;Wang Na;Yang XiaoHong
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）已被用于快速鉴定整个细菌多年。然而，对于液体培养基中培养的细菌的快速鉴定，没有一致的方法。因此，在这项研究中，我们探讨了使用MALDI-TOF MS分析快速鉴定液体培养基中培养的细胞。我们确定2，5-二羟基苯甲酸（50 mg/mL（-1），50%乙腈，0.1%三氟乙酸）是MALDI-TOF MS用于此类研究的最佳基质溶液。此外，测试菌株成功地区分了主成分分析，并找到了混合培养样品中的每个物种的质量峰的主要特征。此外，我们发现用于检测的最小细胞数为1.8x10（3）。总之，我们的研究结果表明，基于MS的技术可以开发作为一种辅助方法，用于快速，准确地识别在液体培养基中培养的细菌。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid identification of bacteria cultured in liquid media. Thus, in this study we explored the use of MALDI-TOF MS analysis for rapid identification of cells cultured in liquid media. We determined that 2,5-dihydroxybenzoic acid (50 mg mL(-1), 50% acetonitrile, 0.1% trifluoroacetic acid) was the best matrix solution for MALDI-TOF MS for this type of study. Moreover, the tested strains were successfully differentiated by principal component analysis, and the main characteristics of the mass peaks for each species were found in mixed culture samples. In addition, we found that the minimum number of cells for detection was 1.8x10(3). In conclusion, our findings suggest that MS-based techniques can be developed as an auxiliary method for rapidly and accurately identifying bacteria cultured in liquid media.