Platelets protect lung from injury induced by systemic inflammatory response
Platelets protect lung from injury induced by systemic inflammatory response
复制标题
血小板保护肺免受全身炎症反应引起的损伤
DOI：
10.1038/srep42080
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发表时间：
2017-02-03
影响因子：
4.6
通讯作者：
Du, Lei
中科院分区：
文献类型：
--
作者：
Luo, Shuhua;Wang, Yabo;Du, Lei
全身性炎症反应可严重损伤肺，促使人们努力探索如何减轻这种损伤。在这里，我们探讨了血小板是否可以帮助减轻由体外循环（ECC）诱导的全身炎症反应引起的小鼠肺损伤。小鼠接受ECC 30分钟，然后用磷酸盐缓冲盐水、血小板、GPIIb/IIIa抑制剂替罗非班或血小板和替罗非班的组合治疗。60 min后收集血液和肺组织，评估肺损伤和炎症状态。正如预期的那样，ECC引起全身炎症和肺功能障碍，血小板输注导致肺损伤明显减轻和肺功能增强。它还导致更多的循环血小板-白细胞聚集体和更多的血小板聚集在肺中。血小板输注与血浆和肺中转化生长因子-β的较高产生以及肿瘤坏死因子-α和中性粒细胞弹性蛋白酶的较低水平相关。在存在替罗非班的情况下未观察到这些血小板效应。我们的研究结果表明，至少在某些条件下，血小板可以保护肺免受全身炎症反应引起的损伤。
Systemic inflammatory responses can severely injure lungs, prompting efforts to explore how to attenuate such injury. Here we explored whether platelets can help attenuate lung injury in mice resulting from extracorporeal circulation (ECC)-induced systemic inflammatory responses. Mice were subjected to ECC for 30 min, then treated with phosphate-buffered saline, platelets, the GPIIb/IIIa inhibitor Tirofiban, or the combination of platelets and Tirofiban. Blood and lung tissues were harvested 60 min later, and lung injury and inflammatory status were assessed. As expected, ECC caused systemic inflammation and pulmonary dysfunction, and platelet transfusion resulted in significantly milder lung injury and higher lung function. It also led to greater numbers of circulating platelet-leukocyte aggregates and greater platelet accumulation in the lung. Platelet transfusion was associated with higher production of transforming growth factor-β and as well as lower levels of tumour necrosis factor-α and neutrophil elastase in plasma and lung. None of these platelet effects was observed in the presence of Tirofiban. Our results suggest that, at least under certain conditions, platelets can protect lung from injury induced by systemic inflammatory responses.