PALINDROMIC UNIT HIGHLY REPETITIVE DNA-SEQUENCES EXHIBIT SPECIES SPECIFICITY WITHIN ENTEROBACTERIACEAE
PALINDROMIC UNIT HIGHLY REPETITIVE DNA-SEQUENCES EXHIBIT SPECIES SPECIFICITY WITHIN ENTEROBACTERIACEAE
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DOI：
10.1016/0923-2508(90)90084-4
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发表时间：
1990-11-01
影响因子：
2.6
通讯作者：
HOFNUNG, M
中科院分区：
文献类型：
--
作者：
GILSON, E;BACHELLIER, S;HOFNUNG, M
回文单位（PU或REP，repeating extragenic palindrome）是由40个核苷酸组成的DNA序列家族，在大肠杆菌基因组中高度重复。我们通过交叉杂交分析了99种不同细菌基因组中PU序列的存在。当PU序列作为探针时，只有与E.大肠杆菌中的宋内志贺菌、鲍氏志贺菌、沙门氏菌、弗氏柠檬酸杆菌和丙二酸左利未杆菌均显示出明显的杂交信号。此外，这些细菌可以分为两组，这对应于它们的PU序列略有不同：E。大肠杆菌群包括S. sonnei和S. boydii和S.鼠伤寒沙门氏菌群包括C. freundii和L.马洛纳蒂卡。
Palindromic units (PU, or REP for repetitive extragenic palindrome) constitute a family of DNA sequences of 40 necleotides which is highly repeated in the genome of Escherichia coli. We analysed the presence of PU sequences in 99 different bacterial genomes by cross-hybridization. When PU sequences were used as a probe, only DNA from Enterobacteriaceae closely related to E. coli exhibited an appreciable hybridization signal: Shigella sonnei, Shigella boydii, Salmonella enteritica serotype Typhimurium, Citrobacter freundii and Levinea malonatica. Furthermore, these bacteria could be divided into two groups which corresponded to a slight difference in their PU sequence: the E. coli group includes S. sonnei and S. boydii; and S. enteritica serotype Typhimurium group includes C. freundii and L. malonatica.