Expression of human poly(ADP-ribose) polymerase with DNA-dependent enzymatic activity in Escherichia coli.
Expression of human poly(ADP-ribose) polymerase with DNA-dependent enzymatic activity in Escherichia coli.
复制标题
具有 DNA 依赖性酶活性的人聚（ADP-核糖）聚合酶在大肠杆菌中的表达。
DOI：
10.1016/0006-291x(89)92285-7
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发表时间：
1989
影响因子：
3.1
通讯作者：
M. Miwa
中科院分区：
文献类型：
--
作者：
M. Ikejima;S. Noguchi;R. Yamashita;H. Suzuki;T. Sugimura;M. Miwa
将人聚（ADP-核糖）聚合酶的 cDNA 插入质粒中，转染并在大肠杆菌中表达。大肠杆菌。 E的裂解物。含有表达质粒的大肠杆菌与抗人聚(ADP-核糖)聚合酶的抗体反应并合成聚(ADP-核糖)。部分纯化的聚（ADP-核糖）聚合酶在大肠杆菌中表达。大肠杆菌与人胎盘聚（ADP-核糖）聚合酶具有相同的分子量和酶学特性，包括对 NAD 的亲和力、周转数和 DNA 依赖性活性。该表达系统应可用于聚（ADP-核糖）聚合酶的结构功能分析。
A cDNA for human poly(ADP-ribose) polymerase was inserted into a plasmid, transfected and expressed inE. coli. A lysate of theE. colicells containing the expression plasmid reacted with antibody against human poly(ADP-ribose) polymerase and synthesized poly(ADP-ribose). The partially purified poly(ADP-ribose) polymerase expressed inE. colihad the same molecular weight and enzymological properties as human placental poly(ADP-ribose) polymerase, including affinity for NAD, turnover number and DNA-dependency for activity. This expression system should be useful for structure-function analysis of poly(ADP-ribose) polymerase.