Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells
Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells
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DOI：
10.3390/nu5051660
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发表时间：
2013-05-01
期刊：
影响因子：
5.9
通讯作者：
Kim, Yangha
中科院分区：
文献类型：
--
作者：
Lee, Mak-Soon;Kim, In-Hwan;Kim, Yangha
解偶联蛋白3(UCP3)是一种线粒体膜转运蛋白，主要在骨骼肌中表达，在能量消耗和脂肪氧化中起重要作用。在本研究中，我们研究了二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对C2C12肌肉细胞UCP3基因表达的影响。EPA和DHA以剂量依赖的方式上调C2C12肌肉细胞中UCP3的mRNA水平，并同样增加UCP3启动子的活性。为了确定AMPK信号是否也可能直接调节UCP3的表达，在C2C12肌肉细胞中处理了激活AMPK的AMP类似物5‘-amino-4-imidazolecarboxamide-ribonucleoside。AICAR与EPA或DHA对UCP3启动子的激活有相加作用。这些结果表明，在C2C12肌肉细胞中，EPA和DHA可能通过AMPK途径直接调节UCP3基因表达。
Uncoupling protein 3 (UCP3) is a mitochondrial membrane transporter that is expressed mainly in skeletal muscle where it plays an important role in energy expenditure and fat oxidation. In this study, we investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on UCP3 gene expression in C2C12 muscle cells. EPA and DHA up-regulated UCP3 mRNA level in a dose-dependent manner and similarly increased UCP3 promoter activity in C2C12 muscle cells. To determine whether AMP-activated protein kinase (AMPK) signaling may also directly regulate UCP3 expression, 5'-amino-4-imidazolecarboxamide-ribonucleoside (AICAR), an AMP analog that activates AMPK, was treated in C2C12 muscle cells. AICAR showed additive effects with EPA or DHA on the UCP3 promoter activation. These results indicate that EPA and DHA directly regulate the gene expression of UCP3, potentially through AMPK-mediated pathway in C2C12 muscle cells.