A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells.
A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells.
复制标题
基于石墨烯纳米材料的新型吸收光谱法检测肝细胞癌特异性T淋巴细胞
DOI：
10.2147/ijn.s168574
复制
发表时间：
2018
影响因子：
8
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Zhu J;Li Y;Li L;Wang J;Wang H;Hong W;Hao K;Xue Y;Chen B;Wang Z
前言抗原特异性细胞毒性T淋巴细胞（CTL）的检测是了解肝癌免疫病理和肝癌免疫治疗的基础。然而，标记CTL的经典方法，主要组织相容性复合物（MHC）-肽四聚体，存在缺陷，需要进一步改进。材料和方法在此，作为一种新的检测探针，基于石墨烯的MHC-肽多聚体被开发用于灵敏地和选择性地识别肝细胞癌特异性T细胞。为了评估其检测效率，还原氧化石墨烯（RGO）用氯化血红素和链霉亲和素官能化以制备官能化HRGO-链霉亲和素复合物。随后将生物素化的MHC-肽单体构建到HRGO上以产生用于CTL标记的检测探针。通过HRGO与四甲基联苯胺之间的反应来检测T细胞的数量。结果HRGO/MHC多肽多聚体在体外诱导系统和患者外周血中均能有效检测到T细胞的数量。结论HRGO/MHC-肽多聚体方法在抗原肽特异性T细胞检测中具有应用前景。
Introduction Detection of antigen-specific cytotoxic T lymphocytes (CTLs) is the foundation for understanding hepatocellular carcinoma immune pathology and hepatocellular carcinoma immunotherapy. However, the classical method for labeling CTLs, major histocompatibility complex (MHC)–peptide tetramer, has drawbacks and needs further improvement. Materials and methods Here, as a new detection probe, a graphene-based MHC–peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO–streptavidin complex. Biotinylated MHC–peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine. Results Using HRGO/MHC–peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients. Conclusion HRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells.