Immunocytochemical mapping of an RDL-like GABA receptor subunit and of GABA in brain structures related to learning and memory in the cricket Acheta domesticus.
Immunocytochemical mapping of an RDL-like GABA receptor subunit and of GABA in brain structures related to learning and memory in the cricket Acheta domesticus.
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RDL 样 GABA 受体亚基和蟋蟀学习和记忆相关大脑结构中 GABA 的免疫细胞化学图谱。
DOI：
10.1101/lm.5.1.78
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发表时间：
1998
影响因子：
2
通讯作者：
A. Strambi
中科院分区：
文献类型：
--
作者：
C. Strambi;M. Cayre;D. B. Sattelle;R. Augier;Pierre Charpin;A. Strambi
用特异性抗血清研究了成年蟋蟀Acheta acuticus脑内RDL样GABA受体和γ-氨基丁酸（GABA）的分布。特别关注的是已知与学习和记忆有关的大脑结构。RDL样GABA受体主要分布在蘑菇体，特别是蘑菇体柄上部和后萼两臂。较弱的免疫染色检测到的远端部分的脚和α和β叶。背侧和腹外侧前脑神经末梢富含RDL样GABA受体。染色也检测到触角叶的肾小球，以及在中央复合体的椭圆体。许多神经元成群的GABA样免疫反应。强烈免疫染色的束支配的花萼和蘑菇体的叶。触角叶的肾小球，椭圆体，以及背侧和腹外侧前脑的神经髓鞘也富含GABA样免疫反应。数据表明GABA样和RDL样GABA受体免疫反应性的分布之间具有良好的相关性。RDL-样GABA受体亚基的显著分布，特别是蘑菇体和触角叶的区域，强调了抑制信号在昆虫大脑这些主要整合中心的信息处理中的重要性。
The distribution of putative RDL-like GABA receptors and of gamma-aminobutyric acid (GABA) in the brain of the adult house cricket Acheta domesticus was studied using specific antisera. Special attention was given to brain structures known to be related to learning and memory. The main immunostaining for the RDL-like GABA receptor was observed in mushroom bodies, in particular the upper part of mushroom body peduncle and the two arms of the posterior calyx. Weaker immunostaining was detected in the distal part of the peduncle and in the alpha and beta lobes. The dorso- and ventrolateral protocerebrum neuropils appeared rich in RDL-like GABA receptors. Staining was also detected in the glomeruli of the antennal lobe, as well as in the ellipsoid body of the central complex. Many neurons clustered in groups exhibit GABA-like immunoreactivity. Tracts that were strongly immunostained innervated both the calyces and the lobes of mushroom bodies. The glomeruli of the antennal lobe, the ellipsoid body, as well as neuropils of the dorso- and ventrolateral protocerebrum were also rich in GABA-like immunoreactivity. The data demonstrated a good correlation between the distribution of the GABA-like and of the RDL-like GABA receptor immunoreactivity. The prominent distribution of RDL-like GABA receptor subunits, in particular areas of mushroom bodies and antennal lobes, underlines the importance of inhibitory signals in information processing in these major integrative centers of the insect brain.
昆虫神经系统中含有生物素的蛋白质，是免疫细胞化学定位程序干扰的潜在来源。
影响因子：
3.8
作者：
Ziegler,R;Engler,DL;Davis,NT
通讯作者：
Davis,NT