CONDUCTIVITY METHOD FOR DETERMINATION OF UREA
CONDUCTIVITY METHOD FOR DETERMINATION OF UREA
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DOI：
10.1021/ac60180a039
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发表时间：
1961-01-01
影响因子：
7.4
通讯作者：
KROONTJE, W
中科院分区：
文献类型：
--
作者：
CHIN, WT;KROONTJE, W
制备含有 C'-乙酰化异烟肼并与 C''-异烟肼和异烟酸组合的标准溶液，这三种物质构成了人尿液和血浆中发现的异烟肼和代谢物的主要部分 (6)。用载体稀释放射性同位素标记的化合物，使得每种代谢物的比活性大致相等。尿液样本含 100 毫克。每毫升。对每毫升含有 10 和 1.0 Mg· 的每种 C“标记的代谢物和血浆样品进行乙酰化异烟肼分析。结果示于表 I。将 C“标记的异烟肼给予人类志愿者和雄性 Sprague Dawley 大鼠。对从这些受试者收集的尿液样本进行乙酰化异烟肼分析。将所得柱流出液冻干，再溶解于少量蒸馏水中，并在丁醇-水(1比1)或异丙醇-水(85比15)展开的What-man 1号滤纸条上进行色谱分析。柱分析前的尿样和含有异烟肼、异烟酸和乙酰化异烟肼的溶液与柱流出物同时运行。由这些条带制成放射自显影图，随后将其进行比色显影。
Standard solutions were prepared containing C “-acetylated isoniazidalone and in combination with C “isoniazid and isonicotinic acid, these three substances comprising the major fraction of isoniazid and metabolites found in human urine and plasma (6). The radioisotopically labeled compounds were diluted with carrier, so that the specific activity was approximately equal for each metabolite. Urine samples con-taining 100 Mg. per ml. of each C “-labeled metabolite and plasma samples containing 10 and 1.0 Mg· per ml. were analyzed for acetylated isoniazid. The results are shown in Table I. C “-labeled isoniazid was administered to both human volunteers and male Sprague Dawley rats. Urine samples collected from these subjects were analyzed for acetylated isoniazid. The column effluent thus obtained was lyophilized, redissolved in a small amount of distilled water, and subjected to chromatographic analysis on What-man No. 1 filter paper strips with de-velopment by butyl alcohol-water (1 to 1) or isopropyl alcohol-water (85 to 15). Urine samples before column analysis and solutions containing isoniazid, isonicotinic acid, and acetylated isoniazid were run simultaneously with the column effluent. Autoradiograms were made of these strips, which were subsequently developed colorimetrically.