Molecular phylogeny and evolution of Caricaceae based on rDNA internal transcribed spacers and chloroplast sequence data
Molecular phylogeny and evolution of Caricaceae based on rDNA internal transcribed spacers and chloroplast sequence data
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DOI：
10.1016/j.ympev.2005.06.017
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发表时间：
2005-11-01
影响因子：
4.1
通讯作者：
Gheysen, G
中科院分区：
文献类型：
--
作者：
Kyndt, T;Van Droogenbroeck, B;Gheysen, G
本研究旨在阐明番木瓜科的系统发育关系和进化。我们的系统发育分析的核糖体DNA和三个叶绿体片段（matk，trnL-trnF，和psbA-trnH）的ITS的核苷酸序列的基础上，包括29个分类群，属于5个属：新热带属Carica，Vasconcellea，Jarilla，和Jacaratia和赤道非洲属Cylicomorpha。有一个相对较低的突变率，matK，和trnL-trnF被用来估计在属的水平上的关系，而属内进化Vasconcellea进行了研究与更多的变量ITS和psbA-trnH序列。在进行最大简约和最大似然分析之前，将编码为二进制字符的空位添加到序列比对中。强调了番木瓜科的单系性、番木瓜属与Vasconcellea属的系统发育距离以及复活的Vasconcellea属的单系性。在Vasconcellea，该科最大的属，可以看出两个得到充分证实的进化谱系：（1）V. x heilbornii，V. weberbaueri，V pativulata和V parviflora和（2）包含该属所有其他分类群的分支。核ITS和叶绿体psbA-trnH数据集之间的不一致，显着引起的一些类群的Vasconcellea，表明网状事件在这个属可能比以前怀疑的更频繁。此外，个体内ITS序列的异质性提供了进一步的证据，杂交或渐渗起源的不同类群和一个假定的杂交属于本属。(c)2005年爱思唯尔公司All rights reserved.
This study focused on clarifying phylogenetic relationships and evolution within Caricaceae. Our phylogenetic analysis based on nucleotide sequences from the ITS of the ribosomal DNA and three chloroplast fragments (matk, trnL-trnF, and psbA-trnH) included 29 taxa belonging to five genera: the neotropical genera Carica, Vasconcellea, Jarilla, and Jacaratia and the equatorial African genus Cylicomorpha. Having a relatively low mutation rate, matK, and trnL-trnF were used for estimating relationships at the generic level, while intrageneric evolution within Vasconcellea was studied with the more variable ITS and psbA-trnH sequences. Gaps, coded as binary characters, were added to the sequence alignments before performing Maximum Parsimony and Maximum Likelihood analyses. Monophyly of Caricaceae as well as phylogenetic distance between Carica and Vasconcellea species, previously belonging to the same genus, and monophyly of the resurrected genus Vasconcellea were emphasized. Within Vasconcellea, the largest genus of this family, two well-confirmed evolutionary lineages could be discerned: (1) V. x heilbornii, V. weberbaueri, V stipulata, and V parviflora and (2) a clade holding all other taxa of the genus. Incongruence between nuclear ITS and chloroplast psbA-trnH datasets, shown to be significantly caused by some taxa of the genus Vasconcellea, indicated that reticulate events in this genus might be more frequent than previously suspected. Moreover, intra-individual ITS sequence heterogeneity provided further evidence for the hybrid or introgressed origin of different taxa and one presumed hybrid belonging to this genus. (c) 2005 Elsevier Inc. All rights reserved.