Characterization of the intramolecular G-quadruplex promoting activity of Est1
Characterization of the intramolecular G-quadruplex promoting activity of Est1
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Est1 分子内 G-四链体促进活性的表征
DOI：
10.1016/j.febslet.2013.01.024
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发表时间：
2013-03-18
期刊：
影响因子：
3.5
通讯作者：
Zhou, Jin-Qiu
中科院分区：
文献类型：
--
作者：
Li, Qian-Jin;Tong, Xia-Jing;Zhou, Jin-Qiu
在芽殖酵母酿酒酵母中，端粒DNA包括TG（1-3）/C（1-3）A双链DNA和突出的富含G的突出端。我们的前期研究表明，端粒酶调节亚基Est 1在体外可促进端粒单链DNA形成分子间G-四链体，而这种活性是端粒复制和保护所必需的。在这项研究中，我们进一步表征了Est 1的G-四链体促进活性。在这里，我们报道了Est 1能够促进模拟天然端粒DNA的单链寡核苷酸（TGTGTGGG）（4）形成分子内G-四链体。因此，它仍然是可能的，分子内G-四链体促进活性的Est 1在端粒复制在体内生物学相关。(C)2013年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
In the budding yeast Saccharomyces cerevisiae, telomeric DNA includes TG(1-3)/C(1-3)A double-stranded DNA and a protruding G-rich overhang. Our previous studies revealed that the telomerase regulatory subunit Est1 promotes telomeric single-stranded DNA to form intermolecular G-quadruplex in vitro, and this activity is required for telomere replication and protection in vivo. In this study, we further characterized the G-quadruplex promoting activity of Est1. Here we report that Est1 is able to promote the single-stranded oligonucleotide of (TGTGTGGG)(4), which mimics the natural telomeric DNA, to form intramolecular G-quadruplex. Therefore, it remains possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo. (C) 2013 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.