OSMOREGULATION IN A NEW HALOALKALIPHILIC BACILLUS FROM THE WADI NATRUN (EGYPT)
OSMOREGULATION IN A NEW HALOALKALIPHILIC BACILLUS FROM THE WADI NATRUN (EGYPT)
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DOI：
10.1016/s0723-2020(85)80003-5
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
TRUPER, HG
中科院分区：
文献类型：
--
作者：
WEISSER, J;TRUPER, HG
从埃及纳特伦河（Wadi Natrun）的一个含碱性、NaCl、硫酸盐和碳酸盐的湖泊中分离到一株新的嗜卤碱芽孢杆菌（Bacillus）。该生物仅在pH 8.5-10之间生长，并且极端耐盐，在含有高达200 g/l NaCl的培养基中生长。在其形态和利用各种底物，它显着不同于以前描述的嗜碱杆菌。细胞内Na+、K+、Cl-、氨基酸和多元醇浓度的测定表明，这些离子负责细胞内的调节。细胞内的NaCl浓度与培养基中的相似。生物体的酶装置似乎不适应高的细胞内离子浓度。所有测试的酶被抑制高浓度的NaCl。
A new haloalkaliphilic Bacillus strain was isolated from an alkaline, NaCl, sulfate and carbonate containing lake of the Wadi Natrun (Egypt). The organism only grows between pH 8.5-10 and is extremely halotolerant, growing in media with up to 200 g/l NaCl. In its morphology and its utilization of various substrates, it differs remarkably from previously described alkaliphilic bacilli. Determination of the intracellular concentration of Na+, K+, Cl-, amino acids and polyols showed that these ions were responsible for osmoregulation. The NaCl concentrations within the cells were similar to those in the medium. The enzymatic apparatus of the organism does not seem to be adapted to the high intracellular ion concentrations. All tested enzymes were inhibited by high concentrations of NaCl.