Genetic linkage map construction and QTL mapping of seedling height, basal diameter and crown width of Taxodium 'Zhongshanshan 302' × T. mucronatum.
Genetic linkage map construction and QTL mapping of seedling height, basal diameter and crown width of Taxodium 'Zhongshanshan 302' × T. mucronatum.
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杉杉 中山山302’‗毛杉遗传连锁图谱构建及苗高、底径、冠宽QTL定位
DOI：
10.1186/s40064-016-2617-3
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Wang Z;Cheng Y;Yin Y;Yu C;Yang Y;Shi Q;Hao Z;Li H
红豆杉是一个以其快速生长，良好的形式和耐洪水，盐，碱性，疾病和强风而闻名的属。本研究利用序列相关扩增多态性（SRAP）和简单重复序列（SSR）标记构建了一张遗传连锁图谱。‘中山山302’和T. mucronatum。该图谱总长度为976.5cM，标记间平均距离为7.0cM，包含34个连锁群和179个标记（171个SRAP和8个SSR）。利用构建的连锁图检测了影响苗高、地径和冠幅等生长性状的数量性状位点。检测到4个显著的QTL，其中3个QTL位于LG 7的2.659 cM处，分别为苗高qtSH-1、地径qtBD-1和冠幅qtCW-1，对数优势值分别为3.72、3.49和3.93，分别解释了3个生长性状总变异的24.9%、27.0%和21.7%。另一个冠幅QTL（qtCW-2）在LG 13上检测到，位于1.0 cM处，优势值的对数为3.15，可解释冠幅总变异的31.7%。这是红豆杉遗传连锁图谱的构建和QTL定位的首次报道，为红豆杉高密度遗传图谱的构建和QTL定位奠定了基础。
Taxodium is a genus renowned for its fast growth, good form and tolerance of flooding, salt, alkalinity, disease and strong winds. In this study, a genetic linkage map was constructed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers based on an F1 population containing 148 individuals generated from a cross between T. ‘Zhongshanshan 302’ and T. mucronatum. The map has a total length of 976.5 cM, with a mean distance of 7.0 cM between markers, and contains 34 linkage groups with 179 markers (171 SRAPs and 8 SSRs). Quantitative trait loci (QTLs) affecting growth traits, such as seedling height, basal diameter and crown width, were detected based on the constructed linkage map. Four significant QTLs were identified, three of which, namely qtSH-1 for seedling height, qtBD-1 for basal diameter and qtCW-1 for crown width, were located at 2.659 cM of LG7 with logarithm odds values of 3.72, 3.49 and 3.93, respectively, and explained 24.9, 27.0 and 21.7 % of the total variation of the three grown traits, respectively. Another QTL for crown width (qtCW-2) was detected at 1.0 cM on LG13, with a logarithm of odds value of 3.15, and explained 31.7 % of the total variation of crown width. This is the first report on the construction of a genetic linkage map and QTL analysis in Taxodium, laying the groundwork for the construction of a high-density genetic map and QTL mapping in the genus Taxodium.