Structural and functional characteristics of irradiation damage to parotid glands in the miniature pig
Structural and functional characteristics of irradiation damage to parotid glands in the miniature pig
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DOI：
10.1016/j.ijrobp.2005.04.029
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发表时间：
2005-08-01
影响因子：
7
通讯作者：
Wang, SL
中科院分区：
文献类型：
--
作者：
Li, J;Shan, ZC;Wang, SL
目的：为探讨大剂量电离辐射（IR）对小型猪腮腺结构和功能的影响，用直线加速器对14只小型猪腮腺进行15或20戈伊剂量照射，另4只小型猪作为非IR对照。在IR前和IR后4周和16周测量唾液流速和唾液化学。还进行了腺体重量和腺泡面积的定量评估以及详细的血清化学和血液学分析。在IR后第4周给予20戈伊或第16周给予15戈伊，腮腺流速降低约50%。在20戈伊组中，在IR后16周，唾液流率减少了约80%。在IR组中发现唾液钙和淀粉酶显著降低，唾液钾水平升高。IR后血清化学和血液学参数也有短暂的变化。IR后4周和16周，15和20戈伊组的腮腺重量显著降低（-50%）。此外，IR组腺体中的腺泡细胞面积在4周和16周的时间点均显著低于对照腺体。唾液腺实质的结构变化发生在IR后相对较早，而唾液分泌量的变化相对延迟。此外，唾液流量的减少与腺泡细胞面积的损失不成比例。总之，这些研究结果表明，非实质IR损伤可能有助于IR诱导的唾液功能减退。(c)2005年爱思唯尔公司
Purpose: To evaluate the effects of a solitary megadose protocol of ionizing radiation (IR) on the structure and function of the miniature pig (minipig) parotid gland.Methods and Materials: Fourteen minipigs were subjected to either 15 or 20 Gy to one parotid gland with a linear accelerator, whereas another four minipigs served as non-IR controls. Salivary flow rates and salivary chemistries were measured pre-IR and 4 and 16 weeks post-IR. A quantitative assessment of gland weight and acinar area and detailed serum chemistry and hematologic analyses were also performed.Results: Parotid flow rates decreased by approximately 50% either with 20 Gy at 4 weeks, or 15 Gy at 16 weeks post-IR. In the 20 Gy group, salivary flow rates were reduced by approximately 80% at 16 weeks post-IR. A significant decrease in salivary calcium and amylase and an increase of salivary potassium levels were found in both IR groups. There were also transient alterations in serum chemistry and hematology parameters post-IR. Parotid gland weights were significantly decreased (-50%) in the 15 and 20 Gy groups at 4 and 16 weeks post-IR. Additionally, the acinar cell area in glands of both IR groups was significantly reduced from that in control glands at both the 4 and 16 weeks time points.Conclusion: Structural changes in salivary gland parenchyma occurred relatively early after IR, whereas the alterations in salivary output were relatively delayed. Further, reductions in salivary flow were not proportional to acinar cell area loss. Together, these findings suggest that nonparenchymal IR damage likely contributes to IR-induced salivary hypofunction. (c) 2005 Elsevier Inc.