Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples
Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples
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DOI：
10.4269/ajtmh.2001.65.362
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发表时间：
2001-10-01
影响因子：
3.3
通讯作者：
Kimura, E
中科院分区：
文献类型：
--
作者：
Itoh, M;Weerasooriya, MV;Kimura, E
我们开发了一种酶联免疫吸附测定 (ELISA)，可检测未浓缩尿液中的丝虫特异性免疫球蛋白 G4 抗体。从患有班氏微丝蚴、抗原或两者兼而有之的人收集的 91 份尿液样本中，有 87 份 (95.6%) ELISA 呈阳性。在泰国、老挝人民民主共和国和日本（这些国家尚未发现人类丝虫病）收集的 298 份尿液样本中，295 份（99.0%）的 ELISA 检测结果呈阴性。各种肠道线虫和吸虫感染均不干扰 ELISA。叠氮化钠尿样可在37℃保存4周，尿液采集时间不影响ELISA结果。该 ELISA 可用于识别丝虫病的地方病灶。
We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%,) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degreesC for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis.