Synthesis of alkyl-ether glycerophospholipids in rat glomerular mesangial cells: evidence for alkyldihydroxyacetone phosphate synthase activity.
Synthesis of alkyl-ether glycerophospholipids in rat glomerular mesangial cells: evidence for alkyldihydroxyacetone phosphate synthase activity.
复制标题
大鼠肾小球系膜细胞中烷基醚甘油磷脂的合成：烷基二羟基丙酮磷酸合酶活性的证据。
DOI：
10.1016/s0006-291x(87)80017-7
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Lianos,EA
中科院分区：
文献类型：
--
作者：
Zanglis,A;Lianos,EA
We studied the ability of rat glomerular mesangial cells and their microsomal fractions to incorporate 1-[14C]hexadecanol to glycerophospholipids via an O-alkyl ether linkage and assessed the presence and activity of the required enzyme: alkyl-dihydroxy acetone phosphate synthase. Suspensions of cultured mesangial cells incorporated 1-[14C]hexadecanol to the phosphatidyl ethanolamine and phosphatidyl choline lipid pools, via a bond resistant to acid and base hydrolysis. When cell homogenates or microsomal fractions were incubated with palmitoyl-DHAP and 1-[14C]hexadecanol, alkyl-DHAP and 1-O-alkyl glycerol were formed (alkyl:hexadecyl). The activity of the enzyme responsible for the O-alkyl product formation was calculated to be 2.5 ± 0.3 and 544 ± 50 pmoles/min/mg protein for mesangial cell homogenates and mesangial cell microsomes, respectively. These observations provide evidence that mesangial cells may elaborate ether linked lipid precursorsde novofor the biosynthesis of O-alkyl glycerophospholipids.
