Anatomical Organization of Urocortin 3-Synthesizing Neurons and Immunoreactive Terminals in the Central Nervous System of Non-Human Primates [Sapajus spp.].
Anatomical Organization of Urocortin 3-Synthesizing Neurons and Immunoreactive Terminals in the Central Nervous System of Non-Human Primates [Sapajus spp.].
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DOI：
10.3389/fnana.2017.00057
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发表时间：
2017
影响因子：
2.9
通讯作者：
Bittencourt JC
中科院分区：
文献类型：
--
作者：
Battagello DS;Diniz GB;Candido PL;da Silva JM;de Oliveira AR;Torres da Silva KR;Lotfi CFP;de Oliveira JA;Sita LV;Casatti CA;Lovejoy DA;Bittencourt JC
尿皮质素3（UCN 3）是促肾上腺皮质激素释放因子（CRF）肽家族的神经肽成员，其充当CRF亚型2（CRF 2）受体的选择性内源性配体。啮齿动物的免疫组织化学和原位杂交数据显示，在中枢神经系统（CNS）的离散区域，如内侧视前核，喙侧穹窿周围区（PFA），杏仁核内侧核和上级旁橄榄核的含UCN 3的神经元。UCN 3-免疫反应性（UCN 3-ir）终末分布在整个区域，大多与CRF 2信使RNA（mRNA）表达区域重叠。目前，对于非人类灵长类动物，还没有类似的映射。为了更好地了解这种神经肽的作用，我们的目的是研究UCN 3在Sapajus属新世界猴大脑中的分布。为此，我们分析了这些动物的基因和肽序列，并进行免疫组织化学和原位杂交，以确定UCN 3的合成位点，并确定UCN 3-IR终端的分布。Sapajus spp. UCN 3编码基因与人和大鼠的同源性分别为88%和65%。此外，使用从猴cDNA产生的探针和抗人UCN 3的抗血清，我们发现标记的细胞主要位于下丘脑和边缘系统区域。UCN 3-ir轴突和终末主要分布在下丘脑腹内侧核（VMH）和外侧隔核（LS）。我们的研究结果表明，UCN 3产生的神经元在中枢神经系统的猴子相比，啮齿类动物的大脑中的神经遗传保守，纤维的分布与CRF 2在其他灵长类动物中的分布一致，并有解剖学证据的参与UCN 3在灵长类动物的神经内分泌控制。
Urocortin 3 (UCN3) is a neuropeptide member of the corticotropin-releasing factor (CRF) peptide family that acts as a selective endogenous ligand for the CRF, subtype 2 (CRF2) receptor. Immunohistochemistry and in situ hybridization data from rodents revealed UCN3-containing neurons in discrete regions of the central nervous system (CNS), such as the medial preoptic nucleus, the rostral perifornical area (PFA), the medial nucleus of the amygdala and the superior paraolivary nucleus. UCN3-immunoreactive (UCN3-ir) terminals are distributed throughout regions that mostly overlap with regions of CRF2 messenger RNA (mRNA) expression. Currently, no similar mapping exists for non-human primates. To better understand the role of this neuropeptide, we aimed to study the UCN3 distribution in the brains of New World monkeys of the Sapajus genus. To this end, we analyzed the gene and peptide sequences in these animals and performed immunohistochemistry and in situ hybridization to identify UCN3 synthesis sites and to determine the distribution of UCN3-ir terminals. The sequencing of the Sapajus spp. UCN3-coding gene revealed 88% and 65% identity to the human and rat counterparts, respectively. Additionally, using a probe generated from monkey cDNA and an antiserum raised against human UCN3, we found that labeled cells are mainly located in the hypothalamic and limbic regions. UCN3-ir axons and terminals are primarily distributed in the ventromedial hypothalamic nucleus (VMH) and the lateral septal nucleus (LS). Our results demonstrate that UCN3-producing neurons in the CNS of monkeys are phylogenetically conserved compared to those of the rodent brain, that the distribution of fibers agrees with the distribution of CRF2 in other primates and that there is anatomical evidence for the participation of UCN3 in neuroendocrine control in primates.
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影响因子：
5.3
作者：
Deussing, Jan M.;Breu, Johannes;Wurst, Wolfgang
通讯作者：
Wurst, Wolfgang
DOI：
10.1073/pnas.1003969107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Kuperman, Yael;Issler, Orna;Chen, Alon
通讯作者：
Chen, Alon
影响因子：
4.8
作者：
Jamieson, Pauline M.;Li, Chien;Vale, Wylie
通讯作者：
Vale, Wylie
影响因子：
2.5
作者：
GATTASS, R;SOUSA, APB;ROSA, MGP
通讯作者：
ROSA, MGP
DOI：
10.1073/pnas.92.3.836
发表时间：
1995-01-31
影响因子：
11.1
作者：
LOVENBERG, TW;LIAW, CW;OLTERSDORF, T
通讯作者：
OLTERSDORF, T