Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
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DOI：
10.1038/s41598-018-37977-7
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发表时间：
2019-02-06
影响因子：
4.6
通讯作者：
Mulder, Klaas W.
中科院分区：
文献类型：
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作者：
Gerlach, Jan P.;van Buggenum, Jessie A. G.;Mulder, Klaas W.
环境刺激往往导致异质细胞反应和转录输出。我们开发了单细胞RNA和免疫检测（RAID），可以对固定单细胞的转录组和细胞内（磷酸化）蛋白进行组合分析。RAID成功地再现了人角质形成细胞在蛋白质和mRNA水平上的分化状态变化。此外，我们发现分化的角质形成细胞保留了高磷酸化的FAK水平，这是与干细胞相关的一个特征，也表达了干细胞相关转录本的选择。我们的数据表明，RAID允许在mRNA和磷酸化蛋白质组水平上研究细胞对环境信号的异质反应。
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.