The fms21 (pilA)-fms20 locus encoding one of four distinct pili of Enterococcus faecium is harboured on a large transferable plasmid associated with gut colonization and virulence.
The fms21 (pilA)-fms20 locus encoding one of four distinct pili of Enterococcus faecium is harboured on a large transferable plasmid associated with gut colonization and virulence.
复制标题
编码屎肠球菌四种不同菌毛之一的 fms21 (pilA)-fms20 基因座位于与肠道定植和毒力相关的大型可转移质粒上。
DOI：
10.1099/jmm.0.016238-0
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发表时间：
2010
影响因子：
3
通讯作者：
Murray,BarbaraE
中科院分区：
文献类型：
--
作者：
Kim,DavidS;Singh,KavindraV;Nallapareddy,SreedharR;Qin,Xiang;Panesso,Diana;Arias,CesarA;Murray,BarbaraE
在过去的二十年中，肠球菌，特别是粪肠球菌和粪肠球菌，已经成为多重耐药的机会性病原体，导致难以治疗的卫生保健相关（HA）感染，包括心内膜炎（Arias & Murray, 2009）。最近的监测报告了一个主要的流行病学转变，即从美国医院分离出粪肠杆菌的频率越来越高（Hidron等人，2008年）。此外，在有多种医疗问题（如癌症、移植或既往手术）的患者中，也有报道称，万古霉素耐药粪肠杆菌菌株导致的发病率和死亡率增加。利用多位点序列分型，流行病学研究表明，HA粪肠杆菌菌株与定植于健康人胃肠道的共生菌株具有不同的序列类型（Top等，2008）。进一步的分析表明，最近成功的粪肠杆菌医院相关基因群[也称为克隆复合体17 (CC17)；Top等人，2008]至少部分是由于编码胶原黏附素的acm基因的活性形式的存在（Nallapareddy等人，2008）或获得额外的基因位点，编码肠球菌表面蛋白（espfm）（Willems等人，2001），透明质酸酶样糖苷水解酶（hylEfm）（Rice等人，2003）或粪肠球菌表面蛋白（fms）（Hendrickx等人，2007；Sillanpaa等人，2008,2009）。菌毛和msmrms（识别粘附基质分子的微生物表面成分）与致病菌粘附和定植宿主组织的能力有关，这是启动感染的重要过程。最近对心内膜源性粪肠杆菌TX0016 （DO）未完成基因组序列的生物信息学分析鉴定出22个Fms蛋白中的15个含有
In the last two decades, enterococci, especially Enterococcus faecalis and Enterococcus faecium, have emerged as multidrug-resistant opportunistic pathogens causing difficult-to-treat healthcare-associated (HA) infections including endocarditis (Arias & Murray, 2009). Recent surveillances reported a major epidemiological shift of increasing frequency of isolation of E. faecium from United States hospitals (Hidron et al., 2008). Furthermore, increased morbidity and mortality due to vancomycin-resistant strains of E. faecium have also been reported in patients who have multiple medical problems, such as cancer, transplantation or prior surgery. Using multilocus sequence typing, epidemiological studies have shown that the HA E. faecium strains are different sequence types from the commensal strains that colonize the gastrointestinal tract of healthy humans (Top et al., 2008). Further analyses suggested that the recent success of the E. faecium hospital-associated genogroup [also referred to as clonal complex 17 (CC17); Top et al., 2008] is due at least in part to the presence of an active form of the acm gene encoding collagen adhesin (Nallapareddy et al., 2008) or acquisition of additional genetic loci encoding enterococcal surface protein (espfm)(Willems et al., 2001), a hyaluronidase-like glycoside hydrolase (hylEfm)(Rice et al., 2003) or E. faecium surface proteins (fms)(Hendrickx et al., 2007; Sillanpaa et al., 2008, 2009).Pili and MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) have been implicated in the ability of pathogenic bacteria to adhere to and colonize host tissues, processes important in initiating infections. Recent bioinformatics analyses of the unfinished genome sequence of endocarditis-derived E. faecium strain TX0016 (DO) identified 15 of 22 Fms proteins containing