A nuclear-encoded chloroplast protein harboring a single CRM domain plays an important role in the Arabidopsis growth and stress response.
A nuclear-encoded chloroplast protein harboring a single CRM domain plays an important role in the Arabidopsis growth and stress response.
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DOI：
10.1186/1471-2229-14-98
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发表时间：
2014-04-16
影响因子：
5.3
通讯作者：
Kang H
中科院分区：
文献类型：
--
作者：
Lee K;Lee HJ;Kim DH;Jeon Y;Pai HS;Kang H
虽然一些叶绿体RNA剪接和核糖体成熟（CRM）结构域的蛋白质已被表征为叶绿体基因表达过程中的内含子剪接和rRNA加工，大多数CRM结构域蛋白在植物生长发育以及叶绿体RNA代谢中的功能作用仍然很大程度上未知。在这里，我们的特点是发展和压力反应的作用，核编码的叶绿体蛋白窝藏一个单一的CRM结构域（At 4g 39040），指定CFM 4，在拟南芥。对CFM 4-GFP融合蛋白的分析表明，CFM 4定位于叶绿体。CFM 4的T-DNA插入功能缺失突变体（cfm 4）表现出生长迟缓和延迟衰老，表明CFM 4在正常生长条件下对植物的生长和发育起作用。此外，cfm 4突变体在胁迫条件下表现出延迟种子萌发和幼苗生长。突变体中叶绿体基因的剪接模式没有发生改变，但16 S和4.5SrRNA的加工发生异常。重要的是，确定CFM 4具有RNA伴侣活性。这些结果表明，叶绿体靶向的CFM 4，两个拟南芥基因编码一个单一的CRM结构域的蛋白质，窝藏RNA分子伴侣活性，并发挥作用，在拟南芥的生长和胁迫反应，通过影响叶绿体中的rRNA加工。
Although several chloroplast RNA splicing and ribosome maturation (CRM) domain-containing proteins have been characterized for intron splicing and rRNA processing during chloroplast gene expression, the functional role of a majority of CRM domain proteins in plant growth and development as well as chloroplast RNA metabolism remains largely unknown. Here, we characterized the developmental and stress response roles of a nuclear-encoded chloroplast protein harboring a single CRM domain (At4g39040), designated CFM4, in Arabidopsis thaliana. Analysis of CFM4-GFP fusion proteins revealed that CFM4 is localized to chloroplasts. The loss-of-function T-DNA insertion mutants for CFM4 (cfm4) displayed retarded growth and delayed senescence, suggesting that CFM4 plays a role in growth and development of plants under normal growth conditions. In addition, cfm4 mutants showed retarded seed germination and seedling growth under stress conditions. No alteration in the splicing patterns of intron-containing chloroplast genes was observed in the mutant plants, but the processing of 16S and 4.5S rRNAs was abnormal in the mutant plants. Importantly, CFM4 was determined to possess RNA chaperone activity. These results suggest that the chloroplast-targeted CFM4, one of two Arabidopsis genes encoding a single CRM domain-containing protein, harbors RNA chaperone activity and plays a role in the Arabidopsis growth and stress response by affecting rRNA processing in chloroplasts.
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影响因子：
11.4
作者：
Ostheimer, GJ;Williams-Carrier, R;Barkan, A
通讯作者：
Barkan, A
DOI：
10.1073/pnas.0407896101
发表时间：
2005-01-04
影响因子：
11.1
作者：
Huang, HR;Rowe, CE;Perlman, PS
通讯作者：
Perlman, PS
影响因子：
4.5
作者：
Barkan, Alice;Klipcan, Larik;Watkins, Kenneth P.
通讯作者：
Watkins, Kenneth P.
影响因子：
4.8
作者：
Keren, Ido;Klipcan, Liron;Ostersetzer-Biran, Oren
通讯作者：
Ostersetzer-Biran, Oren
影响因子：
11.6
作者：
Kim, Won Yong;Jung, Hyun Ju;Kang, Hunseung
通讯作者：
Kang, Hunseung