Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry
Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry
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DOI：
10.1016/j.bbrc.2005.04.129
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Macfarlane, RD
中科院分区：
文献类型：
--
作者：
Farwig, ZN;McNeal, CJ;Macfarlane, RD
血清淀粉样蛋白A（SAA）家族的主要焦点是急性期亚型。然而，组成型异构体（SAA 4）可能对人血清脂蛋白的代谢有很强的影响。在这项研究中，SAA 4蛋白在健康和患病个体的高密度脂蛋白组分中进行了检测。使用超离心结合固相萃取和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测SAA 4的新型异构体。鉴定了三种截短亚型以及两种糖基化亚型。异构体的分布模式可能对心血管风险的评估以及患者治疗的指导具有重要意义。(C)2005年爱思唯尔公司All rights reserved.
The main focus of the serum amyloid A (SAA) family has been on the acute phase isoforms. However, the constitutive isoform (SAA4) may have a strong effect on the metabolism of human serum lipoproteins. In this study, the SAA4 protein was examined in the high-density lipoprotein fraction of both healthy and diseased individuals. Novel isoforms of SAA4 were detected using ultracentrifugation combined with solid-phase extraction and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three truncated isoforms were identified as well as two glycosylated isoforms. Patterns of isoform distribution may be significant for assessment of cardiovascular risk as well as direction of patient treatment. (C) 2005 Elsevier Inc. All rights reserved.