Autoantibodies to thyroglobulin and thyroid peroxidase in blood spots measured with a sensitive, direct radioimmunoassay.
Autoantibodies to thyroglobulin and thyroid peroxidase in blood spots measured with a sensitive, direct radioimmunoassay.
复制标题
使用灵敏的直接放射免疫测定法测量血斑中的甲状腺球蛋白和甲状腺过氧化物酶自身抗体。
DOI：
10.1093/clinchem/36.5.823
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发表时间：
1990
影响因子：
9.3
通讯作者：
K. Beever
中科院分区：
文献类型：
--
作者：
D. Phillips;S. McLachlan;B. Smith;J. Bradbury;K. Beever
The detection of autoantibodies to thyroglobulin(Tg) and thyroid perox-idase (TPO; microsomal antigen) is important in the diagnosis of asymp-tomatic or subclinical autoimmune thyroid disease. Various antibodies can now be measured in dried blood spots, and the use of this method in screening programs has several ad-vantages, in particular the ease of transport and storage of dried blood spots in remote areas of the world lacking laboratory facilities. Thyroid autoantibodies can be detected from eluted blood spots by hemagglutina-tion (1), but little information is avail-able on the sensitivity of measurements performed with more recently developed assays. Because we plan to study these antibodies in iodine-deficient areas in developing countries, we assessed the effect of storage conditions likely to be encountered(high humidity and high ambient temperatures) on the recoverability of autoantibodies in blood spots to be assayed with highly sensitive assays that depend on the direct interaction between ‘25I-labeled antigen and antibody(2). Replicate 20-L aliquots of venous blood from 14 patients known to have Tg and (or) TPO autoantibodies were spotted onto filter paper and air-dried at room temperature for several hours. We punched 6-mm-diameter discs (estimated to contain 11.25 L of whole blood) from the filter papers and eluted the antibody overnight with400 L of assay diluent (per liter, 150 mmol of NaCl, 10 mmol of Tris-HC1, pH 7.5, 5 g of bovine serum albumin, and 1 mL of Tween 20 surfactant). The eluted samples and serum samples from the same patients were analyzed for Tg and TPO antibodies as de-
