Structural and functional changes of immune system in aging mouse induced by D-galactose.
Structural and functional changes of immune system in aging mouse induced by D-galactose.
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发表时间：
2006-12
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通讯作者：
Hongbin Deng;Chun-lei Cheng;D. Cui;Dian-dong Li;L. Cui;N. Cai
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作者：
Hongbin Deng;Chun-lei Cheng;D. Cui;Dian-dong Li;L. Cui;N. Cai
目的探讨D-半乳糖的作用，特别是在衰老过程中免疫系统结构和功能变化中的作用。方法采用ELISA法测定血清晚期糖基化终末产物(AGE)水平。通过透射电子显微镜检测胸腺和脾脏的超微结构。采用MTT法测定淋巴细胞增殖情况。 IL-2活性通过生物测定法测定。 Northern blot检测IL-2 mRNA水平。结果 D-半乳糖（P < 0.01）和 AGE 治疗（P < 0.05）小鼠（n = 8）的血清 AGE 水平显着升高。 D-半乳糖和AGE处理小鼠的胸腺和脾脏超微结构显示出与老年对照组相似的退化变化。脾脏淋巴细胞有丝分裂和IL-2活性也显着降低（P < 0.01，n = 8）。 Northern blot显示IL-2活性的变化是由mRNA表达的变化引起的。然而，与年轻对照组相比，AGE 加氨基胍组这些参数没有显着变化（P < 0.01 或 P < 0.05，n = 8）。结论 D-半乳糖和 AGE 导致体内免疫系统模拟衰老消退变化。
OBJECTIVE To investigate the role of D-galactose, especially in the structural and functional changes of the immune system in aging. METHODS Serum levels of advanced glycation end-products (AGE) were determined by ELISA method. Ultra-structures of thymus and spleen were detected by transmission electron microscopy. MTT method was used to determine the lymphocyte proliferation. IL-2 activity was determined by bioassay. Northern blot was used to detect the IL-2 mRNA levels. RESULTS Serum AGE levels of D-galactose- (P < 0.01) and AGE-treated (P < 0.05) mice (n = 8) were increased significantly. The ultra-structures of thymus and spleen in D-galactose- and AGE-treated mice showed regressive changes similar to those in the aged control group. The lymphocyte mitogenesis and IL-2 activity of spleen were also decreased significantly (P < 0.01, n = 8). The change of IL-2 activity shown by Northern blot resulted from the change of mRNA expression. The AGE plus aminoguanidine group, however, showed no significant change in these parameters in comparison with the young control group (P < 0.01 or P < 0.05, n = 8). CONCLUSION D-galactose and AGE lead to a mimic regression change of aging in the immune system in vivo.