5′-end SAGE for the analysis of transcriptional start sites
5′-end SAGE for the analysis of transcriptional start sites
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DOI：
10.1038/nbt998
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发表时间：
2004-09-01
影响因子：
46.9
通讯作者：
Matsushima, K
中科院分区：
文献类型：
--
作者：
Hashimoto, S;Suzuki, Y;Matsushima, K
mRNA起始位点的鉴定对于建立基因的全长cDNA序列和分析其调控基因表达的启动子区是必不可少的。在这里，我们描述了基因表达的5 '端序列分析（5 SAGE）的发展，可用于全局识别转录起始位点和单个mRNA的频率。在HEK 293人类细胞文库中的25，684个5' SAGE标签中，19，893个与人类基因组匹配。利用RefSeq、UniGene和DBTSS数据库，在基因组的一个基因座中的15，448个标签中，5个SAGE标签中的85.8%-96.1%被分配在mRNA起始位点的-500至+200 nt内。该技术应有助于在多种细胞和组织中进行5 '端转录组分析。
Identification of the mRNA start site is essential in establishing the full-length cDNA sequence of a gene and analyzing its promoter region, which regulates gene expression. Here we describe the development of a 5'-end serial analysis of gene expression (5 SAGE) that can be used to globally identify transcriptional start sites and the frequency of individual mRNAs. Of the 25,684 5' SAGE tags in the HEK293 human cell library, 19,893 matched to the human genome. Among 15,448 tags in one locus of the genome, 85.8%-96.1% of the 5 SAGE tags were assigned within -500 to +200 nt of mRNA start sites using the RefSeq, UniGene and DBTSS databases. This technique should facilitate 5'-end transcriptome analysis in a variety of cells and tissues.