Disproportionate alpha- and beta-mRNA sodium pump subunit content in canine vascular smooth muscle.
Disproportionate alpha- and beta-mRNA sodium pump subunit content in canine vascular smooth muscle.
复制标题
犬血管平滑肌中α-和β-mRNA钠泵亚基含量不成比例。
DOI：
10.1161/01.res.69.1.39
复制
发表时间：
1991
影响因子：
20.1
通讯作者：
Pressley,TA
中科院分区：
文献类型：
--
作者：
Allen,JC;Medford,RM;Zhao,X;Pressley,TA
从犬隐静脉平滑肌细胞中分离的总RNA通过三种日益敏感的方法：北方分析、RNA酶保护试验和聚合酶链反应检测Na+泵α-和β-mRNA含量。北方分析清楚地显示β-mRNA的存在。使用1.5 kb cDNA探针的北方分析或RNA酶保护均未证实α 1信号。然而，一个“α 1样”的转录检测使用聚合酶链反应与两套引物构建到选定的地区的大鼠α 1-cDNA。这些数据表明，犬血管中的β-mRNA大大超过α 1-mRNA。这种差异可能反映在该组织中Na+泵位点的数量相对较少。
Total RNA isolated from canine saphenous vein smooth muscle cells was examined for Na+ pump alpha- and beta-mRNA content by three increasingly sensitive methods: Northern analysis, RNase protection assay, and polymerase chain reaction. Northern analysis clearly showed the presence of beta-mRNA. An alpha 1 signal was not demonstrable using either Northern analysis with a 1.5-kb cDNA probe or RNase protection. However, an "alpha 1-like" transcript was detected using polymerase chain reaction with two sets of primers constructed to selected areas of the rat alpha 1-cDNA. These data suggest that beta-mRNA in canine blood vessels is in large excess of the alpha 1-mRNA. This discrepancy may be reflected in the relatively low number of Na+ pump sites in this tissue.