Comparison of ochratoxin A levels in edible pig tissues and in biological fluids after exposure to a contaminated diet
Comparison of ochratoxin A levels in edible pig tissues and in biological fluids after exposure to a contaminated diet
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DOI：
10.1007/s12550-016-0249-7
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发表时间：
2016-04
影响因子：
3
通讯作者：
J. Pleadin;N. Kudumija;D. Kovačević;G. Scortichini;Salvatore Milone;I. Kmetič
中科院分区：
文献类型：
--
作者：
J. Pleadin;N. Kudumija;D. Kovačević;G. Scortichini;Salvatore Milone;I. Kmetič
本研究的目的是比较动物在4周的育肥期间暴露于污染饲料（250 μg OTA/kg饲料）后猪组织和生物体液中的赭曲霉毒素A（OTA）水平。OTA浓度采用经验证的免疫测定方法（ELISA）和高效液相色谱荧光检测器（HPLC-FD）进行定量。猪组织中最高平均OTA浓度是在暴露动物的肾脏中测定的（13.87 ± 1.41 μg/kg），其次为肺肝脏（10.47 ± 1.97 μg/kg）脾脏（7.28 ± 1.75 μg/kg）（4.81 ± 0.99 μg/kg）、肌肉组织（4.72 ± 0.86 μg/kg）、脂肪组织（4.11 ± 0.88 μg/kg）、心脏（3.71 ± 1.09 μg/kg）和脑（3.01 ± 0.25 μg/kg）。此外，在暴露的最后一天（第28天），与血清（4.77 ± 1.57 μg/L）相比，尿液（16.06 ± 3.09 μg/L）中测定的平均OTA水平显著更高，表明OTA尿液分析可以作为识别人类食用猪组织中该污染物水平升高的良好标志物。这项研究为屠宰场可以使用的猪源性生物材料中最有效的OTA控制提供了指导。
The aim of this study was to compare ochratoxin A (OTA) levels in pig tissues and biological fluids after animal exposure to contaminated diet (250 μg OTA/kg of feed) during 4 weeks of fattening. OTA concentrations were quantified using a validated immunoassay method (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). The highest mean OTA concentration in pig tissues was determined in kidneys of exposed animals (13.87 ± 1.41 μg/kg), followed by lungs (10.47 ± 1.97 μg/kg), liver (7.28 ± 1.75 μg/kg), spleen (4.81 ± 0.99 μg/kg), muscle tissue (4.72 ± 0.86 μg/kg), fat tissue (4.11 ± 0.88 μg/kg), heart (3.71 ± 1.09 μg/kg), and brain (3.01 ± 0.25 μg/kg). Furthermore, on the last day of exposure (day 28), significantly higher mean OTA levels were determined in urine (16.06 ± 3.09 μg/L) in comparison to serum (4.77 ± 1.57 μg/L) showing that OTA urine analysis could be a good marker to identify elevated levels of this contaminant in porcine tissues used for human consumption. This study gave guidelines for the most efficient OTA control in pig-derived biological materials that can be exercised at slaughterhouses.